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Field emission scanning electron microscopy (FE-SEM) as an approach for nanoparticle detection inside cells

SYSNO ASEP	0439433
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Field emission scanning electron microscopy (FE-SEM) as an approach for nanoparticle detection inside cells
Author(s)	Havrdová, M. (CZ) Poláková, K. (CZ) Skopalík, J. (CZ) Vůjtek, M. (CZ) Mokdad, A. (CZ) Homolková, M. (CZ) Tuček, J. (CZ) Nebesářová, Jana (BC-A)_{RID, ORCID} Zbořil, R. (CZ)
Source Title	Micron. - : Elsevier - ISSN 0968-4328 Roč. 67, DEC 2014 (2014), s. 149-154
Number of pages	6 s.
Publication form	Print - P
Language	eng - English
Country	GB - United Kingdom
Keywords	Field emission scanning electronmicroscopy (FE-SEM) ; Stem cells ; Iron oxide nanoparticles ; Cellular morphology ; Endosomes ; Cell uptake
Subject RIV	FD - Oncology ; Hematology
Institutional support	BC-A - RVO:60077344
UT WOS	000343641300019
DOI	10.1016/j.micron.2014.08.001
Annotation	When developing new nanoparticles for bio-applications, it is important to fully characterize the nanoparticle's behavior in biological systems. The most common techniques employed for mapping nanoparticles inside cells include transmission electron microscopy (TEM) and scanning transmission electron microscopy (STEM). These techniques entail passing an electron beam through a thin specimen. STEM or TEM imaging is often used for the detection of nanoparticles inside cellular organelles. However, lengthy sample preparation is required (i.e., fixation, dehydration, drying, resin embedding, and cutting). In the present work, a new matrix (FTO glass) for biological samples was used and characterized by field emission scanning electron microscopy (FE-SEM) to generate images comparable to those obtained by TEM. Using FE-SEM, nanoparticle images were acquired inside endo/lysosomes without disruption of the cellular shape. Furthermore, the initial steps of nanoparticle incorporation into the cells were captured. In addition, the conductive FTO glass endowed the sample with high stability under the required accelerating voltage. Owing to these features of the sample, further analyses could be performed (material contrast and energy-dispersive X-ray spectroscopy (EDS)), which confirmed the presence of nanopartides inside the cells. The results showed that FE-SEM can enable detailed characterization of nanoparticles in endosomes without the need for contrast staining or metal coating of the sample. Images showing the intracellular distribution of nanoparticles together with cellular morphology can give important information on the biocompatibility and demonstrate the potential of nanoparticle utilization in medicine.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2015

Number of the records: 1