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The glycoprotein TRP36 of Ehrlichia sp. UFMG-EV and related cattle pathogen Ehrlichia sp. UFMT-BV evolved from a highly variable clade of E. canis under adaptive diversifying selection
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SYSNO ASEP 0439376 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The glycoprotein TRP36 of Ehrlichia sp. UFMG-EV and related cattle pathogen Ehrlichia sp. UFMT-BV evolved from a highly variable clade of E. canis under adaptive diversifying selection Author(s) Cabezas-Cruz, A. (ES)
Valdés, James J. (BC-A) RID, ORCID
de la Fuente, J. (ES)Source Title Parasites & Vectors. - : BioMed Central - ISSN 1756-3305
Roč. 7, DEC 10 2014 (2014), s. 584Number of pages 7 s. Publication form Online - E Language eng - English Country GB - United Kingdom Keywords Ehrlichia sp. UFMG-EV ; Ehrlichia sp. UFMT-BV ; E. mineirensis ; Host-shift ; Diversifying episodic selection Subject RIV EB - Genetics ; Molecular Biology R&D Projects EE2.3.30.0032 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BC-A - RVO:60077344 UT WOS 000347257500001 DOI 10.1186/s13071-014-0584-5 Annotation Background: A new species of Ehrlichia, phylogenetically distant from E. ruminantium, was found in 2010 infecting cattle in Canada. In 2012 and 2013, we reported the in vitro propagation, molecular and ultrastructural characterization of Ehrlichia sp. UFMG-EV (E. mineirensis), a new species of Ehrlichia isolated from the haemolymph of Brazilian Rhipicephalus (Boophilus) microplus ticks. A new organism, named Ehrlichia sp. UFMT-BV, closely related to Ehrlichia sp. UFMG-EV, was recently described in Brazil and after experimental infection it was shown to be pathogenic for cattle. This new emerging clade of cattle Ehrlichia pathogens is closely related to E. canis. The major immunogenic Tandem Repeat Protein (TRP36; also known as gp36) is extensively used to characterize the genetic diversity of E. canis. Homologs of TRP36 were found in both Ehrlichia sp. UFMG-EV and Ehrlichia sp. UFMT-BV. Findings: Herein, we characterized the evolution of this new Ehrlichia clade using TRP36 sequences. Our working hypothesis is that Ehrlichia sp. UFMG-EV and related microorganisms evolved from a highly variable E. canis clade. In support of our hypothesis we found that Ehrlichia sp. UFMG-EV and Ehrlichia sp. UFMT-BV TRP36 evolved from a highly divergent and variable clade within E. canis and this clade evolved under episodic diversifying selection with a high proportion of sites under positive selection. Conclusion: Our results suggest that Ehrlichia sp. UFMG-EV and Ehrlichia sp. UFMT-BV evolved from a variable clade within E. canis. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2015
Number of the records: 1