Number of the records: 1

Single cell analysis of signaling molecules

SYSNO ASEP	0437862
Document Type	A - Abstract
R&D Document Type	The record was not marked in the RIV
R&D Document Type	Není vybrán druh dokumentu
Title	Single cell analysis of signaling molecules
Author(s)	Klepárník, Karel (UIACH-O)_{RID, ORCID} Luksch, Jaroslav (UIACH-O) Adamová, Eva (UZFG-Y) Potáčová, Anna (UIACH-O) Matalová, E. (CZ) Foret, František (UIACH-O)_{RID, ORCID}
Number of authors	6
Source Title	ITP & LACE 2014. Book of Abstracts. - : Grupo VLS Print Solution, 2014 / Guzman N. A. ; Taveres M. F. M. S. 49-49
Number of pages	1 s.
Publication form	Online - E
Action	International Symposium on Electro- and Liquid Phase-Separation Techniques /21./ and Latin-American Symposium on Biotechnology, Biomedical, Biopharmaceutical, and Industrial Applications of Capillary Electrophoresis and Microchip Technology /21./
Event date	04.10.2014-08.10.2014
VEvent location	Natal
Country	BR - Brazil
Event type	WRD
Language	eng - English
Country	BR - Brazil
Keywords	single cell analysis ; signaling molecules ; caspase
Subject RIV	CB - Analytical Chemistry, Separation
R&D Projects	GA14-28254S GA ČR - Czech Science Foundation (CSF)
Institutional support	UIACH-O - RVO:68081715 ; UZFG-Y - RVO:67985904
Annotation	We have synthesized a probe consisting of CdTe QDs conjugated with oligonucleotide to be used as a donor. Conjugation reaction between carboxylated QDs and aminated oligonucleotides has been performed via zero-length cross-linkers. Sample consisting of clinically-relevant PCR amplicons labelled with ROX (6-carboxyrhodamine) served as acceptor. Upon mutation-specific sample-probe hybridization an increase in fluorescence intensity at 610 nm (the emission spectra maximum of ROX) confirmed a proper function of FRET at a presence of detected mutation. The limit of detection evaluated for PCR amplified fragments of the sample is 4x10-9 M.
Workplace	Institute of Analytical Chemistry
Contact	Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234
Year of Publishing	2015

Number of the records: 1