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Electrophoretic preconcentration and separation of cationic labeled saccharides

SYSNO ASEP	0437860
Document Type	A - Abstract
R&D Document Type	The record was not marked in the RIV
R&D Document Type	Není vybrán druh dokumentu
Title	Electrophoretic preconcentration and separation of cationic labeled saccharides
Author(s)	Partyka, Jan (UIACH-O) Foret, František (UIACH-O)_{RID, ORCID}
Number of authors	2
Source Title	ITP & LACE 2014. Book of Abstracts. - : Grupo VLS Print Solution, 2014 / Guzman N. A. ; Taveres M. F. M. S. 97-97
Number of pages	1 s.
Publication form	Online - E
Action	International Symposium on Electro- and Liquid Phase-Separation Techniques /21./ and Latin-American Symposium on Biotechnology, Biomedical, Biopharmaceutical, and Industrial Applications of Capillary Electrophoresis and Microchip Technology /21./
Event date	04.10.2014-08.10.2014
VEvent location	Natal
Country	BR - Brazil
Event type	WRD
Language	eng - English
Country	BR - Brazil
Keywords	saccharide ; AETMA ; electrophoresis
Subject RIV	CB - Analytical Chemistry, Separation
R&D Projects	GAP301/11/2055 GA ČR - Czech Science Foundation (CSF)
Institutional support	UIACH-O - RVO:68081715
Annotation	In continuation of this work we have been exploring small molecules with permanent positive charge as cationic tags. Oligosaccharides and glycans were labeled by reductive amination and the resulting derivatized saccharides were analyzed by capillary electrophoresis coupled to contactless conductivity detection and electrospray ionization mass spectrometry. The quaternary ammonium based tags assured migration of the oligosaccharide species as cations with electrophoretic mobility independent of the pH of the background electrolyte. For transient isotachophoretic preconcentration, the sample was complemented with ammonium ions and injected as a long zone occupying up to 70% of the separation capillary length. The labeled species, injected in the nanomolar concentrations, were focused into narrow zones by transient isotachophoresis followed by the separation in capillary electrophoretic mode.
Workplace	Institute of Analytical Chemistry
Contact	Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234
Year of Publishing	2015

Number of the records: 1