Possible mechanisms responsible for absence of a retrotransposon family on a plant Y chromosome

Kubát, Zdeněk; Žlůvová, Jitka; Vogel, Ivan; Kováčová, Viera; Čermák, Tomáš; Čegan, Radim; Hobza, Roman; Vyskot, Boris; Kejnovský, Eduard

doi:https://dx.doi.org/10.1111/nph.12669

Number of the records: 1

Possible mechanisms responsible for absence of a retrotransposon family on a plant Y chromosome

1.

SYSNO ASEP	0436683
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Possible mechanisms responsible for absence of a retrotransposon family on a plant Y chromosome
Author(s)	Kubát, Zdeněk (BFU-R)_{RID, ORCID} Žlůvová, Jitka (BFU-R)_{RID, ORCID} Vogel, Ivan (BFU-R) Kováčová, Viera (BFU-R) Čermák, Tomáš (BFU-R) Čegan, Radim (BFU-R)_{RID, ORCID} Hobza, Roman (BFU-R)_{RID, ORCID} Vyskot, Boris (BFU-R)_{RID, ORCID} Kejnovský, Eduard (BFU-R)_{RID, ORCID}
Number of authors	9
Source Title	New Phytologist - ISSN 0028-646X Roč. 202, č. 2 (2014), s. 662-678
Number of pages	17 s.
Publication form	Print - P
Language	eng - English
Country	GB - United Kingdom
Keywords	epigenetics ; genome size ; long terminal repeat
Subject RIV	BO - Biophysics
R&D Projects	GPP501/10/P483 GA ČR - Czech Science Foundation (CSF)
	GAP501/10/0102 GA ČR - Czech Science Foundation (CSF)
	ED1.1.00/02.0068 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	EE2.3.20.0045 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	BFU-R - RVO:68081707
UT WOS	000333060500035
DOI	10.1111/nph.12669
Annotation	Some transposable elements (TEs) show extraordinary variance in abundance along sex chromosomes but the mechanisms responsible for this variance are unknown. Here, we studied Ogre long terminal repeat (LTR) retrotransposons in Silene latifolia, a dioecious plant with evolutionarily young heteromorphic sex chromosomes. Ogre elements are ubiquitous in the S.latifolia genome but surprisingly absent on the Y chromosome. Bacterial artificial chromosome (BAC) library analysis and fluorescence in situ hybridization (FISH) were used to determine Ogre structure and chromosomal localization. Next generation sequencing (NGS) data were analysed to assess the transcription level and abundance of small RNAs. Methylation of Ogres was determined by bisulphite sequencing. Phylogenetic analysis was used to determine mobilization time and selection forces acting on Ogre elements. We characterized three Ogre families ubiquitous in the S.latifolia genome. One family is nearly absent on the Y chromosome despite all the families having similar structures and spreading mechanisms.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2015

Number of the records: 1