Nanostructured diamond layers enhance the infrared spectroscopy of biomolecules

Kozak, Halyna; Babchenko, Oleg; Artemenko, Anna; Ukraintsev, Egor; Remeš, Zdeněk; Rezek, Bohuslav; Kromka, Alexander

doi:https://dx.doi.org/10.1021/la404814c

Number of the records: 1

Nanostructured diamond layers enhance the infrared spectroscopy of biomolecules

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SYSNO ASEP	0436205
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Nanostructured diamond layers enhance the infrared spectroscopy of biomolecules
Author(s)	Kozak, Halyna (FZU-D)_{RID, ORCID} Babchenko, Oleg (FZU-D)_{RID, ORCID} Artemenko, Anna (FZU-D)_{RID, ORCID} Ukraintsev, Egor (FZU-D)_{RID, ORCID} Remeš, Zdeněk (FZU-D)_{RID, ORCID} Rezek, Bohuslav (FZU-D)_{RID, ORCID} Kromka, Alexander (FZU-D)_{RID, ORCID, SAI}
Source Title	Langmuir. - : American Chemical Society - ISSN 0743-7463 Roč. 30, č. 8 (2014), s. 2054-2060
Number of pages	7 s.
Language	eng - English
Country	US - United States
Keywords	nanocrystalline diamond ; optical elements ; nanostructured ; FBS ; GAR-FTIR ; XPS
Subject RIV	BH - Optics, Masers, Lasers
R&D Projects	FR-TI2/736 GA MPO - Ministry of Industry and Trade (MPO)
	GAP108/12/0996 GA ČR - Czech Science Foundation (CSF)
	GPP205/12/P331 GA ČR - Czech Science Foundation (CSF)
	LH12186 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	FZU-D - RVO:68378271
UT WOS	000332494000017
EID SCOPUS	84897732204
DOI	10.1021/la404814c
Annotation	We report on the fabrication and practical use of high-quality optical elements based on Au mirrors coated with diamond layers with flat, nanocolumnar, and nanoporous morphologies. Diamond layers (100 nm thickness) are grown at low temperatures (about 300 °C) from a methane, carbon dioxide, and hydrogen gas mixture by a pulsed microwave plasma system with linear antennas. Using grazing angle reflectance (GAR) Fourier transform infrared spectroscopy with p-polarized light, we compare the IR spectra of fetal bovine serum proteins adsorbed on diamond layers with oxidized (hydrophilic) surfaces. We show that the nanoporous diamond layers provide IR spectra with a signal gain of about 600% and a significantly improved sensitivity limit. This is attributed to its enhanced internal surface area. The improved sensitivity enabled us to distinguish weak infrared absorption peaks of <10-nm-thick protein layers and thereby to analyze the intimate diamond–molecule interface.
Workplace	Institute of Physics
Contact	Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579
Year of Publishing	2015

Number of the records: 1