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Aspergillus pragensis sp nov discovered during molecular reidentification of clinical isolates belonging to Aspergillus section Candidi
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SYSNO ASEP 0436028 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Aspergillus pragensis sp nov discovered during molecular reidentification of clinical isolates belonging to Aspergillus section Candidi Author(s) Hubka, Vít (MBU-M) ORCID
Lysková, P. (CZ)
Frisvad, J.C. (DK)
Peterson, S.W. (US)
Skořepová, M. (CZ)
Kolařík, Miroslav (MBU-M) RID, ORCIDSource Title Medical Mycology. - : Oxford University Press - ISSN 1369-3786
Roč. 52, č. 6 (2014), s. 565-576Number of pages 12 s. Language eng - English Country GB - United Kingdom Keywords Aspergillus candidus ; Aspergillus tritici ; antifungal susceptibility testing Subject RIV EE - Microbiology, Virology R&D Projects EE2.3.20.0055 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EE2.3.30.0003 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support MBU-M - RVO:61388971 UT WOS 000339915400002 DOI 10.1093/mmy/myu022 Annotation The identity of nine clinical isolates recovered from Czech patients and presumptively identified as Aspergillus sp. section Candidi based on colony morphology was revised using sequences of beta-tubulin, calmodulin gene sequence, and internal transcribed spacer rDNA. Six isolates were from suspected and proven onychomycosis, one from otitis externa, and two associated with probable invasive aspergillosis. The results showed that one Aspergillus candidus isolate was the cause of otitis externa, and both isolates obtained from sputa of patients with probable invasive aspergillosis were reidentified as A. carneus (sect. Terrei) and A. flavus (sect. Flavi). Three isolates from nail scrapings were identified as A. tritici, a verified agent of nondermatophyte onychomycosis. One isolate from toenail was determined to be A. candidus and the two isolates belonged to a hitherto undescribed species, Aspergillus pragensis sp. nov. This species is well supported by phylogenetic analysis based on beta-tubulin and calmodulin gene and is distinguishable from other members of sect. Candidi by red-brown reverse on malt extract agar, slow growth on Czapek-Dox agar and inability to grow at 37 degrees C. A secondary metabolite analysis was also provided with comparison of metabolite spectrum to other species. Section Candidi now encompasses five species for which a dichotomous key based on colony characteristics is provided. All clinical isolates were tested for susceptibilities to selected antifungal agents using the Etest and disc diffusion method. Overall sect. Candidi members are highly susceptible to common antifungals. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2015
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