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Zoonotic Dirofilaria repens (Nematoda: Filarioidea) in Aedes vexans mosquitoes, Czech Republic

SYSNO ASEP	0434984
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Zoonotic Dirofilaria repens (Nematoda: Filarioidea) in Aedes vexans mosquitoes, Czech Republic
Author(s)	Rudolf, Ivo (UBO-W)_{RID, ORCID, SAI} Šebesta, Oldřich (UBO-W)_SAI Mendel, Jan (UBO-W)_{RID, SAI, SAI, ORCID} Betášová, Lenka (UBO-W)_{SAI, RID} Bocková, E. (SK) Jedličková, Petra (UBO-W)_{SAI, SAI} Venclíková, Kristýna (UBO-W) Blažejová, Hana (UBO-W)_SAI Šikutová, Silvie (UBO-W)_{RID, SAI, SAI, ORCID} Hubálek, Zdeněk (UBO-W)_{RID, SAI, ORCID}
Number of authors	10
Source Title	Parasitology Research. - : Springer - ISSN 0932-0113 Roč. 113, č. 12 (2014), s. 4663-4667
Number of pages	5 s.
Language	eng - English
Country	DE - Germany
Keywords	Aedes vexans ; Mosquito vectors ; Dirofilaria repens ; Dogs ; Zoonotic dirofilariosis ; Setaria spp.
Subject RIV	EG - Zoology
Institutional support	UBO-W - RVO:68081766
UT WOS	000344866500040
EID SCOPUS	84908302035
DOI	10.1007/s00436-014-4191-3
Annotation	The surveillance of vectors for arthropod-borne pathogens is nowadays an important tool in surveillance programmes throughout Europe. Whereas many studies have been performed to screen arthropods for viruses or bacterial pathogens, only limited information is available concerning the geographical distribution and vector range of pathogenic filariae in Central Europe. To consider the prevalence of filarial parasites in mosquito vectors, we performed a molecular survey of mosquitoes for filarial DNA. Mosquito collection was conducted at six study sites in the South Moravian region (Czech Republic) close to the borders with Slovakia and Austria from 2009 to 2011. Molecular screening of mosquitoes was conducted using conventional PCR with primers designed to amplify the mitochondrial cytochromoxidase subunit I gene as well as the partial 5.8S ribosomal RNA gene. A total of 13,222 mosquitoes belonging to six species were captured and distributed into 237 pools with different numbers of individuals. Overall, four pools were positive for Dirofilaria repens (a minimum infection rate 0.03 %) at two study sites (both natural and urban). Another filarial parasite detected during a study into Aedes vexans mosquitoes revealed the closest homology to Setaria spp. We detected specific D. repens DNA in Ae. vexans mosquitoes for the first time in the Czech Republic and confirmed the circulation of Dirofilaria spp. in a natural focus of infection providing an epidemiological link between autochthonous canine cases and mosquito vectors in the area studied.
Workplace	Institute of Vertebrate Biology
Contact	Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524
Year of Publishing	2015

Number of the records: 1