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Differential Expression and Processing of Matrix Metalloproteinase 19 Marks Progression of Gastrointestinal Diseases
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SYSNO ASEP 0434698 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Differential Expression and Processing of Matrix Metalloproteinase 19 Marks Progression of Gastrointestinal Diseases Author(s) Červinková, Monika (UMG-J)
Horák, P. (CZ)
Kanchev, Ivan (UMG-J) RID
Matej, R. (CZ)
Fanta, J. (CZ)
Sequens, R. (CZ)
Kašpárek, Petr (UMG-J)
Sarnová, Lenka (UMG-J)
Turečková, Jolana (UMG-J) RID
Sedláček, Radislav (UMG-J) RIDSource Title Folia Biologica. - : Univerzita Karlova v Praze - ISSN 0015-5500
Roč. 60, č. 3 (2014), s. 113-122Number of pages 9 s. Language eng - English Country CZ - Czech Republic Keywords matrix metalloproteinase 19 ; macrophages ; colon cancer Subject RIV EB - Genetics ; Molecular Biology R&D Projects GAP302/11/2048 GA ČR - Czech Science Foundation (CSF) GAP303/10/2044 GA ČR - Czech Science Foundation (CSF) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EE.2.3.20.0102 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UMG-J - RVO:68378050 UT WOS 000338479400003 Annotation Matrix metalloproteinases (MMPs), responsible for extracellular matrix remodelling and processing of numerous soluble and cell-surface proteins, appear to play important roles in pathogenesis of gastrointestinal diseases. MMI's influence migration of inflammatory cells, mucosal destruction, matrix deposition and degradation. In this study, we analysed the expression of MMP-19 in the main forms of gastrointestinal diseases including inflammatory bowel diseases (IBD) such as ulcerative coiltis and Crohn's disease, and colorectal carcinoma. We identified prominent MMP-19 expression in unaffected areas of intestinal epithelia and macrophages but not in other cells or tissues. Abundant expression of MMP-19 was also found in the endothelium of blood and lymphatic vessels of inflamed intestinal tissue. High MMP-19 immunoreactivity was also associated with macrophages in inflamed areas and myenteric plexuses. In comparison to the intestinal epithelium, all these cell types and compartments appeared to express MMP-19 irrespective of the disease pathogenesis and progression. Intestinal epithelia exhibited striking differential immunoreactivity for MMP-19. While immunoreactivity of monoclonal antibody recognizing the propeptide domain declined in virtually all IBD and colorectal carcinoma samples, other polyclonal antibodies against the hinge region and propetide domain did not show such an obvious decrease. Additional Western blotting analysis revealed that the antibodies against MMP-19 recognize differently processed forms of this MMP. The disappearance of immunoreactivity of the monoclonal anti-propeptide domain antibody does not mean down-regulation of MMP-19, but processing of the immature form. As this processing likely leads to the activation of this MMP, the differential staining pattern may be an important sign of disease progression. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2015
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