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Peroxidase-like activity of magnetoferritin

SYSNO ASEP	0434307
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Peroxidase-like activity of magnetoferritin
Author(s)	Melníková, V. (CZ) Pospíšková, K. (CZ) Mitróová, Z. (SK) Kopčanský, P. (SK) Šafařík, Ivo (UEK-B)_{RID, SAI}
Source Title	Microchimica Acta. - : Springer - ISSN 0026-3672 Roč. 181, 3-4 (2014), s. 295-301
Number of pages	7 s.
Language	eng - English
Country	AT - Austria
Keywords	magnetoferritin ; magnetic nanoparticles ; peroxidase-like activity ; hydrogen peroxide ; oxidative stress
Subject RIV	CE - Biochemistry
R&D Projects	LD13021 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	RVO:67179843 - RVO:67179843
UT WOS	000330173100002
EID SCOPUS	84892664098
DOI	10.1007/s00604-013-1105-5
Annotation	Magnetoferritin is a spherical biomacromolecule with a diameter of about 12 nm. It consists of a protein shell composed of apoferritin that is surrounding magnetic nanoparticles of magnetite (Fe3O4) or maghemite (gamma-Fe2O3). Magnetoferritins with various iron content (loading factor) were synthetically prepared and their peroxidase-like activities studied via the oxidation of the chromogenic substrate N,N-diethyl-p-phenylenediamine sulfate by hydrogen peroxide to give a purple product with an absorption maximum at 551 nm. Magnetoferritin with higher loading factor exhibits a higher peroxidase-like activity. The catalytic activity was successfully applied to the determination of hydrogen peroxide in the 5.8 to 88.2 mM concentration range.
Workplace	Global Change Research Institute
Contact	Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268
Year of Publishing	2015

Number of the records: 1