Number of the records: 1

Divergence of Borrelia burgdorferi sensu lato spirochetes could be driven by the host: diversity of Borrelia strains isolated from ticks feeding on a single bird

SYSNO ASEP	0432816
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Divergence of Borrelia burgdorferi sensu lato spirochetes could be driven by the host: diversity of Borrelia strains isolated from ticks feeding on a single bird
Author(s)	Rudenko, Natalia (BC-A)_{RID, ORCID} Golovchenko, Maryna (BC-A)_{RID, ORCID} Belfiore, N. M. (US) Grubhoffer, Libor (BC-A)_{RID, ORCID} Oliver, J. H., Jr. (US)
Source Title	Parasites & Vectors. - : BioMed Central - ISSN 1756-3305 Roč. 7, JAN 2014 (2014), s. 4
Number of pages	11 s.
Publication form	Online - E
Language	eng - English
Country	GB - United Kingdom
Keywords	Borrelia burgdorferi sensu lato ; Ixodes minor ; bird migration ; bird reservoir host ; multilocus sequence analysis ; multilocus sequence typing ; recombinant genotypes ; Southeastern United States
Subject RIV	EB - Genetics ; Molecular Biology
Institutional support	BC-A - RVO:60077344
UT WOS	000334628600002
DOI	10.1186/1756-3305-7-4
Annotation	Background: The controversy surrounding the potential impact of birds in spirochete transmission dynamics and their capacity to serve as a reservoir has existed for a long time. The majority of analyzed bird species are able to infect larval ticks with Borrelia. Dispersal of infected ticks due to bird migration is a key to the establishment of new foci of Lyme borreliosis. The dynamics of infection in birds supports the mixing of different species, the horizontal exchange of genetic information, and appearance of recombinant genotypes. Methods: Four Borrelia burgdorferi sensu lato strains were cultured from Ixodes minor larvae and four strains were isolated from Ixodes minor nymphs collected from a single Carolina Wren (Thryothorus ludovicianus). A multilocus sequence analysis that included 16S rRNA, a 5S-23S intergenic spacer region, a 16S-23S internal transcribed spacer, flagellin, p66, and ospC separated 8 strains into 3 distinct groups. Additional multilocus sequence typing of 8 housekeeping genes, clpA, clpX, nifS, pepX, pyrG, recG, rplB, and uvrA was used to resolve the taxonomic status of bird-associated strains. Results: Results of analysis of 14 genes confirmed that the level of divergence among strains is significantly higher than what would be expected for strains within a single species. The presence of cross-species recombination was revealed: Borrelia burgdorferi sensu stricto housekeeping gene nifS was incorporated into homologous locus of strain, previously assigned to B. americana. Conclusions: Genetically diverse Borrelia strains are often found within the same tick or same vertebrate host, presenting a wide opportunity for genetic exchange. We report the cross-species recombination that led to incorporation of a housekeeping gene from the B. burgdorferi sensu stricto strain into a homologous locus of another bird-associated strain.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2015

Number of the records: 1