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Red blood cells serve as intravascular carriers of myeloperoxidase
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SYSNO ASEP 0431577 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Red blood cells serve as intravascular carriers of myeloperoxidase Author(s) Adam, M. (US)
Gajdová, Silvie (BFU-R)
Kolářová, Hana (BFU-R) RID
Kubala, Lukáš (BFU-R) RID, ORCID
Lau, D. (DE)
Geisler, A. (DE)Number of authors 12 Source Title Journal of Molecular and Cellular Cardiology. - : Elsevier - ISSN 0022-2828
Roč. 74, SEP (2014), s. 353-363Number of pages 11 s. Publication form Print - P Language eng - English Country GB - United Kingdom Keywords Myeloperoxidase ; Erythrocyte ; Cell membranes Subject RIV BO - Biophysics R&D Projects GCP305/12/J038 GA ČR - Czech Science Foundation (CSF) Institutional support BFU-R - RVO:68081707 UT WOS 000340077900039 DOI 10.1016/j.yjmcc.2014.06.009 Annotation Myeloperoxidase (MPO) is a heme enzyme abundantly expressed in polymorphonuclear neutrophils. MPO is enzymatically capable of catalyzing the generation of reactive oxygen species (ROS) and the consumption of nitric oxide (NO). Thus MPO has both potent microbicidal and, upon binding to the vessel wall, pro-inflammatory properties. Interestingly, MPO - a highly cationic protein - has been shown to bind to both endothelial cells and leukocyte membranes. Given the anionic surface charge of red blood cells, we investigated binding of MPO to erythrocytes. Red blood cells (RBCs) derived from patients with elevated MPO plasma levels showed significantly higher amounts of MPO by flow cytometry and ELISA than healthy controls. Heparin-induced MPO-release from patient-derived RBCs was significantly increased compared to controls. Ex vivo experiments revealed dose and time dependency for MPO-RBC binding, and immunofluorescence staining as well as confocal microscopy localized MPO-RBC interaction to the erythrocyte plasma membrane. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2015
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