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Counting cell number in situ by quantification of dimethyl sulphide in culture headspace
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SYSNO ASEP 0431395 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Counting cell number in situ by quantification of dimethyl sulphide in culture headspace Author(s) Chippendale, T. W. E. (GB)
Španěl, Patrik (UFCH-W) RID, ORCID
Smith, D. (GB)
El Haj, A. (GB)Source Title Analyst. - : Royal Society of Chemistry - ISSN 0003-2654
Roč. 139, JUL 2014 (2014), s. 4903-4907Number of pages 5 s. Language eng - English Country GB - United Kingdom Keywords TUBE MASS-SPECTROMETRY ; SIFT-MS ; VOLATILE COMPOUNDS Subject RIV CF - Physical ; Theoretical Chemistry Institutional support UFCH-W - RVO:61388955 UT WOS 000341303000023 EID SCOPUS 84906691516 DOI 10.1039/C4AN01102C Annotation A novel, non-invasive technique is reported for determining the numbers of cells in a culture by quantifying dimethyl sulphide (DMS) in the culture headspace as produced by the cellular enzymatic reduction of dissolved dimethyl sulphoxide (DMSO). Measured DMS concentrations, as performed using selected ion flow tube mass spectrometry (SIFT-MS), in the headspace of 2D and 3D cultures of four cell lines, viz. HEK293 (kidney), MG63 (bone), hepG2 (liver) and CALU-1 (lung), linearly correlate with starting cell number. Clear differences in the rates of production of DMS by the four cell types in both the 2D and 3D situations are seen. This novel analytical technique for cell enumeration offers a significant contribution to quality assessment across cell-based research and industry, including analysis of large scale culture systems, and for routine cell biology research. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2015
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