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Towards optical detection of plasmopara viticola infection in the field
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SYSNO ASEP 0430375 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Towards optical detection of plasmopara viticola infection in the field Author(s) Šebela, David (UEK-B) RID, SAI
Olejníčková, Julie (UEK-B) RID, SAI
Sotolář, R. (CZ)
Vrchotová, Naděžda (UEK-B) RID, SAI, ORCID
Tříska, Jan (UEK-B) RID, ORCID, SAISource Title Journal of Plant Pathology - ISSN 1125-4653
Roč. 96, č. 2 (2014), s. 309-320Number of pages 12 s. Language eng - English Country IT - Italy Keywords Downy mildew ; grapevine ; UV-induced fluorescence ; chlorophyll fluorescence ; reflectance ; transresveratrol ; 2,4,6-trihydroxyphenanthrene-2-O-glucoside Subject RIV EH - Ecology, Behaviour R&D Projects ED1.1.00/02.0073 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support RVO:67179843 - RVO:67179843 UT WOS 000348436800011 EID SCOPUS 84903944280 DOI 10.4454/JPP.V96I2.037 Annotation Chlorophyll fluorescence, UV-induced fluorescence and reflectance signals of grapevine leaf tissue naturally infected by Plasmopara viticola were inspected in three susceptible grapevine cultivars (Cabernet Sauvignon, Pinot Blanc and Pinot Gris). Distribution of FV/FM parameter over the leaf was found to be effective to discriminate symptomatic and asymptomatic leaf tissue. Reduction of FV/FM parameter ~ 25% was found in all infected leaf spots. Infected leaves also expressed significantly changed chlorophyll fluorescence induction kinetics expressing much slower electron transport rate on donor and acceptor site of photosystem II. Symptomatic leaves emitted high fluorescence signal under UV excitation 320nm, centered at 395nm, emanated mainly from highly fluorescent compound 2,4,6-trihydroxyphenanthrene-2-O- glucoside. Increase of this compound was found in all inspected grapevine varieties, while its concentration was strongly dependent on resveratrol concentration. Concentration of trans- resveratrol ranged in asymptomatic leaves from 0.1μg·g-1 (CS) to 0.2μg·g-1 (PB, PG). Highest increase of trans-resveratrol in infected leaf tissue was found in PB (4.4μg·g-1), followed by CS (1.6μg·g-1) and PG (1.1μg·g-1). 2,4,6-trihydroxyphenanthrene-2-O-glucoside concentrations varied from 0.41 (PB), 0.75 (PG) to 1.01μg·g-1 (CS) in asymmptomatic leaves, since its concentration rapidly increase with severity of infection [71.09 (PB), 79.37 (PG), and 26.14 μg·g- 1 (CS)]. Reflectance vegetation indices SRI R750/R700, (R780-R710)/(R780-R680) and CRI700 were the most powerful to follow changes in chlorophylls and carotenoids contents under Plasmopara viticola infection. Infected leaves also exhibited lower chlorophyll a (~50 %) and carotenoids (~70%) content. Combination of these optical signals can be used as an effective, non-invasive tool for an early detection of Plasmopara viticola in field. Workplace Global Change Research Institute Contact Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Year of Publishing 2015
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