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Evaluation of antibacterial properties of novel phthalocyanines against Escherichia coli - comparison of analytical methods

SYSNO ASEP	0430204
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Evaluation of antibacterial properties of novel phthalocyanines against Escherichia coli - comparison of analytical methods
Author(s)	Mikula, Přemysl (BU-J) Kalhotka, L. (CZ) Jančula, Daniel (BU-J)_RID Zezulka, Štěpán (BU-J)_ORCID Kořínková, R. (CZ) Černý, J. (CZ) Maršálek, Blahoslav (BU-J)_{ORCID, SAI} Toman, Petr (UMCH-V)_{RID, ORCID}
Number of authors	8
Source Title	Journal of Photochemistry and Photobiology. B - Biology Section. - : Elsevier - ISSN 1011-1344 Roč. 138, Sep 2014 (2014), s. 230-239
Number of pages	10 s.
Language	eng - English
Country	CH - Switzerland
Keywords	phthalocyanines ; photosensitizers ; antibacterial effect
Subject RIV	EF - Botanics
Subject RIV - cooperation	Institute of Macromolecular Chemistry - Macromolecular Chemistry
R&D Projects	TA01010356 GA TA ČR - Technology Agency of the Czech Republic (TA ČR)
	FR-TI3/196 GA MPO - Ministry of Industry and Trade (MPO)
	GAP205/10/2280 GA ČR - Czech Science Foundation (CSF)
Institutional support	BU-J - RVO:67985939 ; UMCH-V - RVO:61389013
UT WOS	000340987500027
EID SCOPUS	8490374045
DOI	10.1016/j.jphotobiol.2014.04.014
Annotation	We analyzed antibacterial effects of several novel phthalocyanines against E. coli and evaluated the suitability of flow cytometry for the detection of antibacterial effects of phthalocyanines in comparison with routinely used cultivation. After 3 hours of exposure under cool white light eight cationic phthalocyanines showed very high antibacterial activity in the concentration of 2.00 mg L-1 and four of them were even efficient in the concentration of 0.20 mg L-1. Antibacterial activity of neutral and anionic compounds was considerably lower or even negligible. Agglomeration of cells observed in suspensions treated with a higher concentration of certain cationic phthalocyanines affected cytometric measurements of total cell counts, therefore this parameter seems not to be fully valid in the evaluation of phthalocyanine antibacterial activity. On the other hand, cytometric measurement of cell membrane integrity appears to be a suitable and sensitive parameter.
Workplace	Institute of Botany
Contact	Martina Bartošová, martina.bartosova@ibot.cas.cz, ibot@ibot.cas.cz, Tel.: 271 015 242 ; Marie Jakšová, marie.jaksova@ibot.cas.cz, Tel.: 384 721 156-8
Year of Publishing	2015

Number of the records: 1