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Comprehensive portrait of cholesterol containing oxidized membrane

SYSNO ASEP	0427792
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Comprehensive portrait of cholesterol containing oxidized membrane
Author(s)	Štefl, Martin (UFCH-W) Šachl, Radek (UFCH-W)_{RID, ORCID} Olžyńska, Agnieszka (UFCH-W)_RID Amaro, Mariana (UFCH-W)_{RID, ORCID} Savchenko, Dariia (FZU-D)_{RID, ORCID} Deyneka, Alexander (FZU-D) Hermetter, A. (AT) Cwiklik, Lukasz (UFCH-W)_{RID, ORCID} Humpolíčková, Jana (UFCH-W)_RID Hof, Martin (UFCH-W)_{RID, ORCID}
Source Title	Biochimica Et Biophysica Acta-Biomembranes. - : Elsevier - ISSN 0005-2736 Roč. 1838, č. 7 (2014), s. 1769-1776
Number of pages	8 s.
Language	eng - English
Country	NL - Netherlands
Keywords	oxidized lipids ; lateral diffusion ; molecular dynamics simulations
Subject RIV	CF - Physical ; Theoretical Chemistry
R&D Projects	GBP208/12/G016 GA ČR - Czech Science Foundation (CSF)
	LH13259 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UFCH-W - RVO:61388955 ; FZU-D - RVO:68378271
UT WOS	000336695300009
EID SCOPUS	84899037032
DOI	10.1016/j.bbamem.2014.02.006
Annotation	Biologicalmembranes are under significant oxidative stress caused by reactive oxygen species mostly originating during cellular respiration. Double bonds of the unsaturated lipids are most prone to oxidation,which might lead to shortening of the oxidized chain and inserting of terminal either aldehyde or carboxylic group. Structural rearrangement of oxidized lipids, addressed already, is mainly associated with looping back of the hydrophilic terminal group. This contribution utilizing dual-focus fluorescence correlation spectroscopy and electron paramagnetic resonance as well as atomistic molecular dynamics simulations focuses on the overall changes of the membrane structural and dynamical properties once it becomes oxidized. Particularly, attention is paid to cholesterol rearrangement in the oxidized membrane revealing its preferable interaction with carbonyls of the oxidized chains. In this view cholesterol seems to have a tendency to repair, rather than condense, the bilayer.
Workplace	J. Heyrovsky Institute of Physical Chemistry
Contact	Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
Year of Publishing	2015

Number of the records: 1