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Comparison of Protein Fractions in Seminal Plasma from Multiple Sperm Collections in Sterlet (Acipenser ruthenus)

SYSNO ASEP	0425839
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Comparison of Protein Fractions in Seminal Plasma from Multiple Sperm Collections in Sterlet (Acipenser ruthenus)
Author(s)	Shaliutina, A. (CZ) Hulák, M. (CZ) Li, P. (CZ) Šulc, Miroslav (MBU-M)_{RID, ORCID} Dzyuba, B. (CZ) Linhart, O. (CZ)
Source Title	Reproduction in Domestical Animals. - : Wiley - ISSN 0936-6768 Roč. 48, č. 1 (2013), s. 156-159
Number of pages	4 s.
Language	eng - English
Country	DE - Germany
Keywords	TROUT ONCORHYNCHUS-MYKISS ; SEMEN CHARACTERISTICS ; SPERMATOZOA
Subject RIV	EE - Microbiology, Virology
Institutional support	MBU-M - RVO:61388971
UT WOS	000313875800027
DOI	10.1111/j.1439-0531.2012.02118.x
Annotation	Contents Seminal plasma of sterlet Acipenser ruthenus was evaluated using comparative proteomics to characterize its protein fractions and to determine any influence of multiple sperm collections on these proteins. An experimental group of fish was used, in which sperm was collected three times at 5 h intervals. Protein fractions of seminal plasma were determined by SDS-gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis high-resolution gels (2D). At all stripping times, five protein bands with molecular weights of 93, 53, 48, 33 and 28 kDa were identified using SDS-PAGE. No significant differences (p > 0.05) in relative mass of protein bands among collections were observed. At the third collection, 20 protein spots were detected from the two-dimensional gels, compared to 17 found at the first and second collections. Ten protein spots, from the third stripping, were analysed
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2014

Number of the records: 1