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Gadolinium- and manganite-based contrast agents with fluorescent probes for both magnetic resonance and fluorescence imaging of pancreatic islets: a comparative study
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SYSNO ASEP 0425115 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Gadolinium- and manganite-based contrast agents with fluorescent probes for both magnetic resonance and fluorescence imaging of pancreatic islets: a comparative study Author(s) Berková, Z. (CZ)
Jirák, D. (CZ)
Zacharovová, K. (CZ)
Lukeš, I. (CZ)
Kotková, Z. (CZ)
Kotek, J. (CZ)
Kačenka, M. (CZ)
Kaman, Ondřej (FZU-D) RID, ORCID
Řehoř, I. (CZ)
Hájek, M. (CZ)
Saudek, F. (CZ)Source Title ChemMedChem. - : Wiley - ISSN 1860-7179
Roč. 8, č. 4 (2013), s. 614-621Number of pages 8 s. Language eng - English Country DE - Germany Keywords contrast agents ; gadolinium ; magnetic resonance imaging ; manganite ; pancreatic islets Subject RIV BM - Solid Matter Physics ; Magnetism Institutional support FZU-D - RVO:68378271 UT WOS 000316811100010 EID SCOPUS 84875627754 DOI 10.1002/cmdc.201200439 Annotation Three magnetic resonance (MR)/fluorescence imaging probes were tested for visualization, cellular distribution, and survival of labeled pancreatic islets invitro and following transplantation. As T1 contrast agents (CAs), Gd(III) complexes linked to -cyclodextrin (Gd-F-CD) or bound to titanium dioxide (TiO2@RhdGd) were tested. As a T2 CA, perovskite manganite nanoparticles (LSMO@siF@si) were examined. Fluorescein or rhodamine was incorporated as a fluorescent marker in all probes. Islets labeled with Gd(III) CAs were visible as hyperintense spots on MR invitro, but detection invivo was inconclusive. Islets labeled with LSMO@siF@si CA were clearly visible as hypointense spots or areas on MR scans invitro as well as invivo. All CAs were detected inside the islet cells by fluorescence. Although the vitality and function of the labeled islets was not impaired by any of the tested CAs, results indicate that LSMO@siF@si CA is a superior marker for islet labeling. Workplace Institute of Physics Contact Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579 Year of Publishing 2014
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