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Simple power supply for power load controlled isoelectric focusing

SYSNO ASEP	0425057
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Simple power supply for power load controlled isoelectric focusing
Author(s)	Duša, Filip (UIACH-O)_{RID, ORCID} Šlais, Karel (UIACH-O)_{RID, ORCID}
Number of authors	2
Source Title	Electrophoresis. - : Wiley - ISSN 0173-0835 Roč. 35, č. 8 (2014), s. 1114-1117
Number of pages	4 s.
Language	eng - English
Country	DE - Germany
Keywords	isoelectric focusing ; power supply ; voltage multiplier
Subject RIV	CB - Analytical Chemistry, Separation
R&D Projects	VG20102015023 GA MV - Ministry of Interior (MV)
Institutional support	UIACH-O - RVO:68081715
UT WOS	000334126600005
DOI	10.1002/elps.201300518
Annotation	The power supply for IEF based on features of the Cockcroft-Walton voltage multiplier (CW VM) is described in this work. The article describes a design of the IEF power supply, its electric characteristics, and testing by IEF analysis. A circuit diagram of the power supply included two opposite charged branches (each consisting of four voltage doublers). The designed CW VM was powered by 230 V/50 Hz alternate current and it generated up to 5 kV and 90 mW at the output. Voltage and current characteristics of the power supply were measured by known load resistances in the range from 10 k to 1 G , which is a common resistance range for IEF strip geometry. Further, the power supply was tested by a separation of a model mixture of colored pI markers using a 175 × 3 × 0.5 mm focusing bed. Automatically limited power load enabled analysis of samples without previous optimization of the focusing voltage or electric current time courses according to sample composition. Moreover, the developed power supply did not produce any intrinsic heat and was easy to set up with cheap and commonly available parts.
Workplace	Institute of Analytical Chemistry
Contact	Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234
Year of Publishing	2015
Electronic address	http://hdl.handle.net/11104/0231022

Number of the records: 1