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Multidimensional separation methods in analysis of complex biological mixtures
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SYSNO ASEP 0424665 Document Type D - Thesis R&D Document Type The record was not marked in the RIV Title Multidimensional separation methods in analysis of complex biological mixtures Author(s) Duša, Filip (UIACH-O) RID, ORCID Number of authors 1 Issue data Brno: Masaryk university, Faculty of Science, 2014 Number of pages 92 s. Publication form Print - P Language eng - English Country CZ - Czech Republic Keywords isoelectric focusing ; liquid chromatography ; two-dimensional separations ; sample pretreatment ; proteins Subject RIV CB - Analytical Chemistry, Separation R&D Projects EE2.3.20.0182 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UIACH-O - RVO:68081715 Annotation This work deals with two-dimensional separations of peptides and proteins from biological matrices. Theoretical introduction describes extraction of proteins from biological matrices, modes of isoelectric focusing (IEF) and liquid chromatography (LC) used for fractionation in two-dimensional separation schemes, and current papers dealing with combination of IEF and LC. In the results chapter there are four papers, which passed reviews and have been published in international scientific journals. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2014
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