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Extracellular Enzymes of the White-Rot Fungus Fomes fomentarius and Purification of 1,4-beta-Glucosidase
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SYSNO ASEP 0423807 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Extracellular Enzymes of the White-Rot Fungus Fomes fomentarius and Purification of 1,4-beta-Glucosidase Author(s) Větrovský, Tomáš (MBU-M) ORCID, RID
Baldrian, Petr (MBU-M) RID, ORCID
Gabriel, Jiří (MBU-M) RIDSource Title Applied Biochemistry and Biotechnology. - : Springer - ISSN 0273-2289
Roč. 169, č. 1 (2013), s. 100-109Number of pages 10 s. Language eng - English Country US - United States Keywords Cellulose ; 1,4-beta-glucosidase ; Glycosyl hydrolase Subject RIV EE - Microbiology, Virology R&D Projects LA10001 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) OC08050 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support MBU-M - RVO:61388971 UT WOS 000314023100009 DOI 10.1007/s12010-012-9952-9 Annotation Production of the lignocellulose-degrading enzymes endo-1,4-beta-glucanase, 1,4-beta-glucosidase, cellobiohydrolase, endo-1,4-beta-xylanase, 1,4-beta-xylosidase, Mn peroxidase, and laccase was characterized in a common wood-rotting fungus Fomes fomentarius, a species able to efficiently decompose dead wood, and compared to the production in eight other fungal species. The main aim of this study was to characterize the 1,4-beta-glucosidase produced by F. fomentarius that was produced in high quantities in liquid stationary culture (25.9 U ml(-1)), at least threefold compared to other saprotrophic basidiomycetes, such as Rhodocollybia butyracea, Hypholoma fasciculare, Irpex lacteus, Fomitopsis pinicola, Pleurotus ostreatus, Piptoporus betulinus, and Gymnopus sp. (between 0.7 and 7.9 U ml(-1)). The 1,4-beta-glucosidase enzyme was purified to electrophoretic homogeneity by both anion-exchange and size-exclusion chromatography Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2014
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