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Novel human multiple myeloma cell line UHKT-893

SYSNO ASEP	0423264
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Novel human multiple myeloma cell line UHKT-893
Author(s)	Uherková, L. (CZ) Vančurová, I. (CZ) Vyhlídalová, I. (CZ) Pleschnerová, M. (CZ) Špička, I. (CZ) Mihalová, R. (CZ) Březinová, J. (CZ) Hodný, Zdeněk (UMG-J)_RID Čermáková, K. (CZ) Polanská, V. (CZ) Marinov, I. (CZ) Jedelský, P.L. (CZ) Kuželová, K. (CZ) Stöckbauer, P. (CZ)
Source Title	Leukemia Research. - : Elsevier - ISSN 0145-2126 Roč. 37, č. 3 (2013), s. 320-326
Number of pages	7 s.
Publication form	Print - P
Language	eng - English
Country	GB - United Kingdom
Keywords	human myeloma cell line ; human multiple myeloma ; plasma cell ; IL-6 dependence ; immunoglobulin ; free light chain
Subject RIV	EB - Genetics ; Molecular Biology
Institutional support	UMG-J - RVO:68378050
UT WOS	000314871500017
DOI	10.1016/j.leukres.2012.12.004
Annotation	We established and characterized a new IL-6 dependent multiple myeloma (MM) cell line UHKT-893 from the bone marrow of a relapsed 57-year-old woman. Using nephelometry, cells with plasma cell phenotype and morphology were found to secrete IgG and free kappa (κ)-light chain of immunoglobulin. κ-Light chain was also recognized intracellularly by flow cytometry and by mass spectrometry. VH4-39 region of IgVH genes was rearranged and somatically hypermutated. Cytogenetic analysis of cells revealed new chromosome abnormalities in all breakpoints unique in both MM patients and cell lines – t(1;6), t(1;11), t(5;15), t(5;21), +der(11;15) and der(16). IL-6 independent subline UHKT-893a was established by adaptation to descending IL-6 concentration, while the original cell line keeps on maintaining its IL-6 dependency. The cell line provides a suitable material for cellular and molecular studies of tumor abnormalities, with potentially unique mutagenic features of myeloma disease. It may be utilized for human hybridoma construction and vaccine development. Both IL-6 dependent and independent cell clones represent an important model for studies of myeloma cell growth and resistance emerging during targeted therapy.
Workplace	Institute of Molecular Genetics
Contact	Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
Year of Publishing	2014

Number of the records: 1