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Molecular analysis of the TGF-beta controlled gene expression program in chicken embryo dermal myofibroblasts
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SYSNO ASEP 0423219 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Molecular analysis of the TGF-beta controlled gene expression program in chicken embryo dermal myofibroblasts Author(s) Kosla, Jan (UMG-J) RID
Dvořák, Michal (UMG-J) RID
Čermák, Vladimír (UMG-J)Source Title Gene. - : Elsevier - ISSN 0378-1119
Roč. 513, č. 1 (2013), s. 90-100Number of pages 11 s. Language eng - English Country NL - Netherlands Keywords microarray ; myofibroblastic phenotype ; inhibition of TGF-beta signaling Subject RIV EB - Genetics ; Molecular Biology R&D Projects KAN200520801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) Institutional support UMG-J - RVO:68378050 UT WOS 000314385700012 DOI 10.1016/j.gene.2012.10.069 Annotation The myofibroblast is a mesenchymal cell characterized by synthesis of the extracellular matrix, plus contractile and secretory activities. Myofibroblasts participate in physiological tissue repair, but can also cause devastating fibrosis. They are present in the tumor stroma of carcinomas and contribute to tumor growth and spreading. As myofibroblasts derive from various cell types and appear in a variety of tissues, there is marked variability in their phenotype. As regulatory mechanism of wound healing are likely conserved among vertebrates, detailed knowledge of these mechanisms in more distant species will help to distinguish general from specific phenomena. To provide this as yet missing comparison, we analyzed the impact of the chemical inhibition of TGF-beta signaling on gene expression in chicken embryo dermal myofibroblasts. We revealed genes previously reported in mammalian systems (e.g. SPON2, ASPN, COMP, LUM, HAS2, IL6, CXCL12,VEGFA) as well as novel TGF-beta dependent genes, among them PGF, VEGFC, PTN, FAM180A, FIBIN, ZIC1, ADCY2, RET, HHIP and DNER. Inhibition of TGF-beta signaling also induced multiple genes, including NPR3, AGTR2, MTUS1, SOD3 and NOV. We also analyzed the effects of long term inhibition, and found that it is not able to induce myofibroblast dedifferentiation. (c) 2012 Elsevier B.V. All rights reserved. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2014
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