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High pressure modification of the simple automated liquid chromatographic system for splitless nano column gradient separations
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SYSNO ASEP 0422993 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title High pressure modification of the simple automated liquid chromatographic system for splitless nano column gradient separations Author(s) Šesták, Jozef (UIACH-O) ORCID
Kahle, Vladislav (UIACH-O) RIDNumber of authors 2 Source Title Chemické Listy. Roč. 107, Issue s3.. - Praha : Česká společnost chemická, 2013 - ISSN 0009-2770 Pages s. 438-440 Number of pages 3 s. Publication form Online - E Action CECE Junior 2013 Event date 12.11.2013-13.11.2013 VEvent location Brno Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords liquid chromatography ; nano column gradient separations ; gradient separation Subject RIV CB - Analytical Chemistry, Separation R&D Projects VG20112015021 GA MV - Ministry of Interior (MV) Institutional support UIACH-O - RVO:68081715 UT WOS 000328730800063 Annotation Our simple liquid chromatographic system for splitless gradient nanocolumn separations at backpressure up to 50 bars was modified to run analysis under the pressure up to 300 bars. Programmable syringe pump was equipped with a 250-L high-pressure syringe and mobile phase gradients were created and stored in relatively long capillary. Available linear gradients of volumes from 5 to 50 L traced by uracil are presented. Sample introduction was performed as separate step and real large volume of sample (tens of microliters) could be injected and preconcentrated on-column. Relative standard deviation of retention times and peak areas were received (RSD < 0.3 % and RSD < 5 % respectively). Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2014
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