Porous layer open tubular columns with immobilized trypsin for protein digestion

Number of the records: 1

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SYSNO ASEP	0422787
Document Type	C - Proceedings Paper (int. conf.)
R&D Document Type	Conference Paper
Title	Porous layer open tubular columns with immobilized trypsin for protein digestion
Author(s)	Knob, R. (CZ) Křenková, Jana (UIACH-O)_{RID, ORCID} Petr, J. (CZ) Foret, František (UIACH-O)_{RID, ORCID}
Number of authors	4
Source Title	Chemické Listy. Roč. 107, Issue s3.. - Praha : Česká společnost chemická, 2013 - ISSN 0009-2770
Pages	s. 382-384
Number of pages	3 s.
Publication form	Online - E
Action	CECE Junior 2013
Event date	12.11.2013-13.11.2013
VEvent location	Brno
Country	CZ - Czech Republic
Event type	WRD
Language	eng - English
Country	CZ - Czech Republic
Keywords	monolith ; enzymes ; digestion
Subject RIV	CB - Analytical Chemistry, Separation
R&D Projects	EE2.3.20.0182 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UIACH-O - RVO:68081715
UT WOS	000328730800041
Annotation	We have developed a monolithic porous layer open tubular (PLOT) column with immobilized trypsin for protein digestion. The PLOT column was prepared in a 10 (mikro)m ID fused silica capillary. Trypsin was immobilized on the monolithic surface and the developed enzyme reactor was used for protein digestion followed by on-line ESI/MS analysis.
Workplace	Institute of Analytical Chemistry
Contact	Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234
Year of Publishing	2014

Number of the records: 1