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Chemoenzymatic synthesis of alpha-L-rhamnosides using recombinant alpha-L-rhamnosidase from Aspergillus terreus

SYSNO ASEP	0422632
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Chemoenzymatic synthesis of alpha-L-rhamnosides using recombinant alpha-L-rhamnosidase from Aspergillus terreus
Author(s)	De Winter, K. (BE) Šimčíková, Daniela (MBU-M) Schlack, B. (BE) Weignerová, Lenka (MBU-M)_RID Pelantová, Helena (MBU-M)_{ORCID, RID} Soetaert, W. (BE) Desmet, T. (BE) Křen, Vladimír (MBU-M)_{RID, ORCID}
Source Title	Bioresource Technology. - : Elsevier - ISSN 0960-8524 Roč. 147, NOV 2013 (2013), s. 640-644
Number of pages	5 s.
Language	eng - English
Country	NL - Netherlands
Keywords	alpha-L-Rhamnosidase ; Aspergillus terreus ; Glycosylation
Subject RIV	CE - Biochemistry
R&D Projects	LD13042 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	7E11011 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	MBU-M - RVO:61388971
UT WOS	000326660900088
DOI	10.1016/j.biortech.2013.08.083
Annotation	This study describes an efficient, large scale fermentation of a recombinant alpha-L-rhamnosidase originating from Aspergillus terreus. High-cell-density Pichia pastoris fermentation resulted in yields up to 627 U/L/h. The recombinant enzyme was used for the reverse rhamnosylation of various small organic compounds. A full factorial experimental design setup was applied to identify the importance of temperature, substrate concentrations, solvent type and concentration as well as the acidity of the reaction mixture. Careful optimization of these parameters allowed the synthesis of a range of alpha-L-rhamnosides among which cyclohexyl alpha-L-rhamnopyranoside, anisyl alpha-L-rhamnopyranoside and 2-phenylethyl alpha-L-rhamnopyranoside. In addition, alpha-L-rhamnosylation of phenolic hydroxyls in phenols such as hydroquinone, resorcinol, catechol and phenol was observed, which is a rather unique reaction catalyzed by glycosidases
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2014

Number of the records: 1