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Optimization of N-glycopeptides analysis methods and their preliminary application to barley proteins study

SYSNO ASEP	0422562
Document Type	C - Proceedings Paper (int. conf.)
R&D Document Type	Conference Paper
Title	Optimization of N-glycopeptides analysis methods and their preliminary application to barley proteins study
Author(s)	Benkovská, Dagmar (UIACH-O) Flodrová, Dana (UIACH-O) Bobálová, Janette (UIACH-O)_{RID, ORCID} Laštovičková, Markéta (UIACH-O)_{RID, ORCID}
Number of authors	4
Source Title	Chemické Listy. Roč. 107, Issue s3.. - Praha : Česká společnost chemická, 2013 - ISSN 0009-2770
Pages	s. 296-298
Number of pages	3 s.
Publication form	Online - E
Action	CECE Junior 2013
Event date	12.11.2013-13.11.2013
VEvent location	Brno
Country	CZ - Czech Republic
Event type	WRD
Language	eng - English
Country	CZ - Czech Republic
Keywords	glycosylation ; post-translational modification ; barley ; N-glycopetptides
Subject RIV	CB - Analytical Chemistry, Separation
R&D Projects	EE2.3.20.0182 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GPP503/12/P395 GA ČR - Czech Science Foundation (CSF)
Institutional support	UIACH-O - RVO:68081715
UT WOS	000328730800005
Annotation	N-glycosylation is the most frequently studied plant protein post-translational modification. The analysis of Nglycopeptides after protein proteolytic digestion offers information about the structure of both oligosaccharide and peptide moiety. However, this method has so far been less commonly used. Since glycopeptides hardly ionize during MS analysis in the presence of non-glycosylated peptides, they need to be separated from the complex peptide mixture. In this study, the glycopeptides enrichment, purification and analysis methods were successfully optimized on two standard N-glycoproteins. Concanavalin A (ConA) lectin tips were used for glycopeptide capturing, and obtained fractions were purified on carbon tips and analyzed using MALDI-TOF mass spectrometry. The differences in the CID fragmentation of certain types of glycopeptides were found. This technique was then applied to glycopeptide analysis of barley grain and malt proteins. Several barley glycopeptides were found, however, their identification was very difficult. More proteins separation techniques will be required before this enrichment procedure in further studies.
Workplace	Institute of Analytical Chemistry
Contact	Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234
Year of Publishing	2014

Number of the records: 1