Number of the records: 1

Evaluation of DNA bending models in their capacity to predict electrophoretic migration anomalies of satellite DNA sequences

SYSNO ASEP	0422254
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Evaluation of DNA bending models in their capacity to predict electrophoretic migration anomalies of satellite DNA sequences
Author(s)	Matyášek, Roman (BFU-R)_ORCID Fulneček, Jaroslav (BFU-R) Kovařík, Aleš (BFU-R)_{RID, ORCID}
Number of authors	3
Source Title	Electrophoresis. - : Wiley - ISSN 0173-0835 Roč. 34, č. 17 (2013), s. 2511-2521
Number of pages	11 s.
Publication form	Print - P
Language	eng - English
Country	US - United States
Keywords	HIGHLY REPETITIVE DNA ; DOUBLE-HELICAL DNA ; CURVED DNA
Subject RIV	AC - Archeology, Anthropology, Ethnology
R&D Projects	GA206/09/1751 GA ČR - Czech Science Foundation (CSF)
	GAP501/10/0208 GA ČR - Czech Science Foundation (CSF)
	GA13-10057S GA ČR - Czech Science Foundation (CSF)
Institutional support	BFU-R - RVO:68081707
CEZ	AV0Z50040702 - BFU-R (2007-2013)
UT WOS	000327667000007
EID SCOPUS	84883310801
DOI	10.1002/elps.201300227
Annotation	DNA containing a sequence that generates a local curvature exhibits a pronounced retardation in electrophoretic mobility. Various theoretical models have been proposed to explain relationship between DNA structural features and migration anomaly. Here, we studied the capacity of 15 static wedge-bending models to predict electrophoretic behavior of 69 satellite monomers derived from four divergent families. All monomers exhibited retarded mobility in PAGE corresponding to retardation factors ranging 1.02-1.54. The curvature varied both within and across the groups and correlated with the number, position, and lengths of A-tracts. Two dinucleotide models provided strong correlation between gel mobility and curvature prediction; two trinucleotide models were satisfactory while remaining dinucleotide models provided intermediate results with reliable prediction for subsets of sequences only. In some cases, similarly shaped molecules exhibited relatively large differences in mobility and vice versa.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2014

Number of the records: 1