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Humoral immune response and spreading of Encephalitozoon cuniculi infection in experimentally infected ponies

SYSNO ASEP	0421260
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Humoral immune response and spreading of Encephalitozoon cuniculi infection in experimentally infected ponies
Author(s)	Wagnerová, Pavla (BC-A) Sak, Bohumil (BC-A)_{RID, ORCID} Květoňová, Dana (BC-A)_SAI Maršálek, M. (CZ) Langerová, I. (CZ) Kváč, Martin (BC-A)_{RID, RID, ORCID}
Source Title	Veterinary Parasitology. - : Elsevier - ISSN 0304-4017 Roč. 197, 1-2 (2013), s. 1-6
Number of pages	6 s.
Publication form	Print - P
Language	eng - English
Country	NL - Netherlands
Keywords	Ponies ; Encephalitozoon cuniculi genotype II ; PCR ; Antibodies ; Experimental infection
Subject RIV	EC - Immunology
Institutional support	BC-A - RVO:60077344
UT WOS	000324787400001
DOI	10.1016/j.vetpar.2013.05.007
Annotation	A total of 9 (8 stallions and 1 mare) 1 year old ponies were used for the experimental infection caused by Encephalitozoon cuniculi genotype II (107 spores per animal). Subsequently, individual horses were slaughtered 7, 14, 21, 28, 35, 42, 49, 56, and 63 days post infection. Immediately after slaughter, tissues samples of stomach, duodenum, jejunum, ileum, caecum, colon, spleen, liver, kidney, bladder, heart, lungs, and brain were sampled. In addition, urine, feces and blood specimens were collected. Enzyme-linked immunosorbent assay was used for determination of humoral immune response and nested PCR targeting 16S rDNA, whole ITS, and 5.8S rDNA was used for detection of E. cuniculi in collected organs, blood, feces and urine. No clinical signs of microsporidiosis including diarrhea or colic, neurological signs and fever were observed in any horses during whole experiment. Acute microsporidiosis in ponies was characterized by the dissemination of microsporidia into almost all organs and significant increase of concentration of specific antibodies in blood was observed from 28 to 42 DPI. After this acute stage microsporidia disappeared from most organs with the exception of the kidney, which was positive up to 63 DPI when the experiment was terminated. No pathological changes were observed in any organs with exception of one mare's brain, where E. cuniculi-positive cavity measuring 5cm x 3 cm in diameter formed in the lobus pinformis.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2014

Number of the records: 1