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Study of Streptavidin-Modified Quantum Dots by Capillary Electrophoresis

  1. 1.
    SYSNO ASEP0421227
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleStudy of Streptavidin-Modified Quantum Dots by Capillary Electrophoresis
    Author(s) Stanisavljevic, M. (CZ)
    Janů, L. (CZ)
    Šmerková, K. (CZ)
    Křížková, S. (CZ)
    Pizúrová, Naděžda (UFM-A) RID, ORCID
    Ryvolová, M. (CZ)
    Adam, V. (CZ)
    Hubálek, J. (CZ)
    Kizek, R. (CZ)
    Number of authors9
    Source TitleChromatographia - ISSN 0009-5893
    Roč. 76, 7-8 (2013), s. 335-343
    Number of pages9 s.
    Languageeng - English
    CountryDE - Germany
    KeywordsCapillary electrophoresis ; Gel electrophoresis ; Avidin-biotin technology ; Oligonucleotide ; Nanoparticle ; quantum dots
    Subject RIVCE - Biochemistry
    Institutional supportUFM-A - RVO:68081723
    UT WOS000316819600006
    DOI10.1007/s10337-012-2372-8
    AnnotationGreat boom of nanotechnologies impacts almost all areas of science and therefore detail understanding of the properties of nanomaterials as well as their interaction abilities is required. Surface modification and functionalization of nanoparticles is of a great interest due to the wide range of applications in the area of nanomedicine, nanobiology, and/or biochemistry. In this study, CdTe QDs were synthesized using microwave reactor and their surface was modified by streptavidin to ensure further suitability for bioconjugation with biotin-labelled oligonucleotides. For characterization of the synthesized QDs and for monitoring of the interaction with the oligonucleotide, capillary and gel electrophoresis was used.
    WorkplaceInstitute of Physics of Materials
    ContactYvonna Šrámková, sramkova@ipm.cz, Tel.: 532 290 485
    Year of Publishing2014
Number of the records: 1  

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