Number of the records: 1

Quantification of active caspases in stem cells: single cell analysis at femtogram level

SYSNO ASEP	0399403
Document Type	A - Abstract
R&D Document Type	The record was not marked in the RIV
R&D Document Type	Není vybrán druh dokumentu
Title	Quantification of active caspases in stem cells: single cell analysis at femtogram level
Author(s)	Adamová, Eva (UIACH-O) Lišková, Marcela (UIACH-O) Klepárník, Karel (UIACH-O)_{RID, ORCID} Hampl, Aleš (UEM-P) Matalová, Eva (UZFG-Y)_RID
Number of authors	5
Source Title	The Abstracts of the World Conference on Regenerative Medicine 2013. - Leipzig : Fraunhofer Institute for Cell Therapy and Immunology, 2013 S. 358
Number of pages	1 s.
Publication form	Online - E
Action	World Conference on Regenerative Medicine 2013 /WCRM 2013/
Event date	23.10.2013-25.10.2013
VEvent location	Leipzig
Country	DE - Germany
Event type	WRD
Language	eng - English
Country	DE - Germany
Keywords	caspases ; cell analysis ; cancer cell
Subject RIV	CB - Analytical Chemistry, Separation
Subject RIV - cooperation	Institute of Animal Physiology and Genetics - Cell Biology Institute of Experimental Medicine - Genetics ; Molecular Biology
R&D Projects	GAP206/11/2377 GA ČR - Czech Science Foundation (CSF)
	GAP304/11/1418 GA ČR - Czech Science Foundation (CSF)
Institutional support	UIACH-O - RVO:68081715 ; UZFG-Y - RVO:67985904 ; UEM-P - RVO:68378041
Annotation	There are several methods for caspase detection in tissue sections and individual cells. However, quantification lags behind qualitative evaluation of active caspases. Therefore, first we aimed to develop/modify a precise technique for active caspase measurement, and second to apply the procedure to express amount of active caspase per cell in cell populations. Further, we improved the method for single cell analysis. As one of model systems, stem cells (neural crest derived) were selected and challenged by camptothecin, a cytotoxic alkaloid used as natural anticancer drug.
Workplace	Institute of Analytical Chemistry
Contact	Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234
Year of Publishing	2014

Number of the records: 1