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Effect of Selected Monosaccharide on Growth and Putrescine Production of Serratia marcescens
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SYSNO ASEP 0398831 Document Type J - Journal Article R&D Document Type The record was not marked in the RIV Subsidiary J Ostatní články Title Effect of Selected Monosaccharide on Growth and Putrescine Production of Serratia marcescens Author(s) Pleva, P. (CZ)
Lazárková, Z. (CZ)
Andresová, Adéla (UCHP-M) RID, SAI
Lorencová, E. (CZ)
Buňka, F. (CZ)
Buňková, L. (CZ)Source Title Plasty a kaučuk - ISSN 0322-7340
Roč. 28, SI (2012)Number of pages 3 s. Language eng - English Country CZ - Czech Republic Keywords monosaccharides ; chromatography ; microbial metabilic activity Subject RIV CF - Physical ; Theoretical Chemistry Institutional support UCHP-M - RVO:67985858 Annotation Putrescine is low-molecular nitrogenous compound that is formed in foodstuffs mainly by microbial decarboxylation of the precursor-amino acid ornithine. The aim of the study was to describe the effect of glucose, galactose and fructose content (0.00, 0.25, 0.50, 0.75, and 1.00% w/v) and pH(6, 7 and 8) on growth and decarboxylase activity of Serratia marcescens. Putrescine production was detemined using the ion-exchange chromatography with post-column ninhydrine derivatization and spectrophotometric detection. While the optimal pH for bacteria growth was 7-8, the decarboxylases showed maximal activity at pH 6. Monosaccharides stimulated both bacteria growth and putrescine formation at pH 6. On the contrary, addition of all monosaccharides tested caused inhibition of putrescine production in Serratia marcescens at pH 7 and 8. Workplace Institute of Chemical Process Fundamentals Contact Eva Jirsová, jirsova@icpf.cas.cz, Tel.: 220 390 227 Year of Publishing 2014
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