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Towards crystal structures of antibodies and transcription factors

SYSNO ASEP	0398525
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Towards crystal structures of antibodies and transcription factors
Author(s)	Písačková, Jana (UOCHB-X)_ORCID Procházková, Kateřina (UOCHB-X) Král, Vlastimil (UMG-J)_RID Fábry, Milan (UMG-J)_RID Řezáčová, Pavlína (UOCHB-X)_{RID, ORCID}
Number of authors	5
Source Title	Materials Structure in Chemistry, Biology, Physics and Technology. - : Czech and Slovak Crystallographic Association - ISSN 1211-5894 Roč. 20, č. 2 (2013), s. 121-123
Number of pages	3 s.
Language	eng - English
Country	CZ - Czech Republic
Keywords	scFv antibody fragment ; DeoR repressor protein ; thermofluor assay ; protein crystallization
Subject RIV	FR - Pharmacology ; Medidal Chemistry
R&D Projects	ME08016 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	1M0505 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA203/09/0820 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z40550506 - UOCHB-X (2005-2011)
	AV0Z50520514 - UMG-J (2005-2011)
Annotation	Protein three-dimensional structure determination by X-ray crystallography is important for understanding protein function. The protein crystallization bottleneck can be overcome by optimization of factors which influence the protein crystallizability. We demonstrated this approach on two scFv antibody fragments, and on prokaryotic metabolic regulator. We have performed protein pre-crystallization analysis employing the combination of size-exclusion chromatography, dynamic light scattering, and thermofluor assay. Based on the analysis data, we have developed crystallization protocols which yielded monocrystals suitable for X-ray diffraction analysis. This enabled us to determine crystal structure of the effector-binding domain of DeoR repressor.
Workplace	Institute of Organic Chemistry and Biochemistry
Contact	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
Year of Publishing	2014

Number of the records: 1