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Measurement of DNA base and nucleotide excision repair activities in mammalian cells and tissues using the comet assay - A methodological overview

SYSNO ASEP	0398229
Document Type	J - Journal Article
R&D Document Type	The record was not marked in the RIV
Subsidiary J	Článek ve WOS
Title	Measurement of DNA base and nucleotide excision repair activities in mammalian cells and tissues using the comet assay - A methodological overview
Author(s)	Azqueta, A. (ES) Langie, S. A. S. (GB) Slyšková, Jana (UEM-P)_RID Collins, A. R. (NO)
Number of authors	4
Source Title	Dna Repair. - : Elsevier - ISSN 1568-7864 Roč. 12, č. 11 (2013), s. 1007-1010
Number of pages	4 s.
Language	eng - English
Country	NL - Netherlands
Keywords	comet assay ; base excision repair ; nucleotide excision repair
Subject RIV	EB - Genetics ; Molecular Biology
Institutional support	UEM-P - RVO:68378041
UT WOS	000327579200017
EID SCOPUS	84887183019
DOI	10.1016/j.dnarep.2013.07.011
Annotation	The article is a comprehensive summary of protocols for the comet-based BER- and NER-specific in vitro DNA repair assays that can be applied to a wide spectrum of biological material - cultured cell lines, blood cells, animal tissue samples and human biopsies. Our intention was to provide a detailed and user-friendly account of the assays, including practical tips and recommendations to help in setting them up in order to facilitate comparison of results obtained in different laboratories.
Workplace	Institute of Experimental Medicine
Contact	Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218
Year of Publishing	2014

Number of the records: 1