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Comparison of myosin heavy chain mRNAs, protein isoforms and fiber type proportions in the rat slow and fast muscles
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SYSNO ASEP 0396901 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Comparison of myosin heavy chain mRNAs, protein isoforms and fiber type proportions in the rat slow and fast muscles Author(s) Žurmanová, J. (CZ)
Soukup, Tomáš (FGU-C) RIDSource Title Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408
Roč. 62, č. 4 (2013), s. 445-453Number of pages 9 s. Language eng - English Country CZ - Czech Republic Keywords rat ; soleus ; extensor digitorum longus ; myosin heavy chain isoforms ; muscle gene expression ; quantitative Real Time RT-PCR ; SDS-PAGE ; fiber type analysis Subject RIV EA - Cell Biology R&D Projects GA304/08/0256 GA ČR - Czech Science Foundation (CSF) GA305/09/1228 GA ČR - Czech Science Foundation (CSF) 7AMB12SK158 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LH12058 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support FGU-C - RVO:67985823 CEZ AV0Z50110509 - FGU-C (2005-2011) UT WOS 000323510500012 EID SCOPUS 84883522567 Annotation We studied the expression of myosin heavy chain isoforms at mRNA and protein levels as well as fiber type composition in the fast extensor digitorum longus (EDL) and slow soleus (SOL) twitch muscles of adult inbred Lewis strain rats. Based on our data presenting quantitative evidence of corresponding proportions between mRNA level, protein content and fiber type composition, we suggest that the Real Time RT-PCR technique can be used as a routine method for analysis of muscle composition changes and could be advantageous for the analysis of scant biological samples such as muscle biopsies in humans Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2014
Number of the records: 1