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Heterosubtypic protection against influenza A induced by adenylate cyclase toxoids delivering conserved HA2 subunit of hemagglutinin

SYSNO ASEP	0396067
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Heterosubtypic protection against influenza A induced by adenylate cyclase toxoids delivering conserved HA2 subunit of hemagglutinin
Author(s)	Staneková, Z. (SK) Adkins, Irena (MBU-M) Kosová, Martina (MBU-M) Janulíková, J. (SK) Šebo, Peter (MBU-M)_{RID, ORCID} Varečková, E. (SK)
Source Title	Antiviral Research. - : Elsevier - ISSN 0166-3542 Roč. 97, č. 1 (2013), s. 24-35
Number of pages	11 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Bordetella adenylate cyclase toxoid ; Influenza A infection ; Cross-protection
Subject RIV	FR - Pharmacology ; Medidal Chemistry
R&D Projects	GA310/08/0447 GA ČR - Czech Science Foundation (CSF)
	GP310/09/P582 GA ČR - Czech Science Foundation (CSF)
Institutional support	MBU-M - RVO:61388971
UT WOS	000314332000003
DOI	10.1016/j.antiviral.2012.09.008
Annotation	The protective efficacy of currently available influenza vaccines is restricted to vaccine strains and their close antigenic variants. A new strategy to obtain cross-protection against influenza is based on conserved antigens of influenza A viruses (IAV), which are able to elicit a protective immune response. Here we describe a vaccination approach involving the conserved stem part of hemagglutinin, the HA2 subunit, shared by different HA subtypes of IAV. To increase its immunogenicity, a novel strategy of antigen delivery to antigen presenting cells (APCs) has been used. The HA2 segment (residues 23-185) was inserted into a genetically detoxified adenylate cyclase toxoid (CyaA-E5) which specifically targets and penetrates CD11b-expressing dendritic cells. The CyaA-E5-HA2 toxoid induced HA2(93-102), HA2(96-104) and HA2(170-178)-specific and Th1 polarized T-cell responses, and also elicited strong broadly cross-reactive HA2-specific antibody response. BALB/c mice immunized with three doses of purified CyaA-E5-HA2 without any adjuvant recovered from influenza infection 2 days earlier than the control mock-immunized mice
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2014

Number of the records: 1