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An efficient proteomic approach to analyze agriculture crop biomass

SYSNO ASEP	0395793
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	An efficient proteomic approach to analyze agriculture crop biomass
Author(s)	Flodrová, Dana (UIACH-O) Bobálová, Janette (UIACH-O)_{RID, ORCID}
Number of authors	2
Source Title	Protein Journal. - : Springer - ISSN 1572-3887 Roč. 32, č. 5 (2013), s. 365-372
Number of pages	8 s.
Publication form	Print - P
Language	eng - English
Country	DK - Denmark
Keywords	MALDI ; biomass ; proteomics ; identification ; hemicellulases
Subject RIV	CB - Analytical Chemistry, Separation
R&D Projects	1M0570 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UIACH-O - RVO:68081715
UT WOS	000321625500005
DOI	10.1007/s10930-013-9495-0
Annotation	While a plant cell wall is formed by a complex of various components, including polysaccharides and structural proteins, its composition and representation may vary during cell growth. Currently, plant research targets the proteins participating in wall loosening. Multiple classes of enzymes, including various hemicellulases and cellulases, are required for plant material degradation to achieve the maximum decomposition. Identifying the set of proteins involved in the breakdown of cell-wall polymers is important to understand plant material conversion into suitable products. The objective of this study was to describe a method which can be used to carry out proteomics analysis of complex plant samples and identify enzymes degrading biomass. For this purpose we used proteomic techniques including gel electrophoresis, high pressure liquid chromatography combinated with mass spectrometry followed by data evaluation using databases searching. Results show that more than 50 % of these activities correspond to enzymes with proteolytic function. This study was focused primarily on enzymes able to breakdown the lignocellulosic and hemicellulosic parts that are very important for the material conversion into required products of degradation.
Workplace	Institute of Analytical Chemistry
Contact	Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234
Year of Publishing	2014

Number of the records: 1