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Determination of antibiotics in influents and effluents of wastewater-treatment-plants in the Czech Republic – development and application of the SPE and a UHPLC-ToFMS method
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SYSNO ASEP 0395780 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Determination of antibiotics in influents and effluents of wastewater-treatment-plants in the Czech Republic – development and application of the SPE and a UHPLC-ToFMS method Author(s) Tylová, Tereza (MBU-M)
Flieger, Miroslav (MBU-M) ORCID
Olšovská, Jana (MBU-M)Source Title Analytical Methods: advancing methods and applications . - : Royal Society of Chemistry - ISSN 1759-9660
Roč. 5, č. 8 (2013), s. 2110-2118Number of pages 9 s. Language eng - English Country GB - United Kingdom Keywords TANDEM MASS-SPECTROMETRY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; ANTIMICROBIAL CONSUMPTION ESAC Subject RIV CB - Analytical Chemistry, Separation R&D Projects 1M06011 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support MBU-M - RVO:61388971 UT WOS 000316960200031 DOI 10.1039/c3ay00048f Annotation A method consisting of solid phase extraction followed by ultra-high performance liquid chromatography combined with time-of-flight mass spectrometry (UHPLC-ToFMS) has been developed for the determination of multiple-class antibiotics in influents and effluents of wastewater-treatment-plants (WWTPs). The nineteen analyzed antibiotics belong to the 5 most prescribed antibiotic classes in the Czech Republic, namely tetracyclines, macrolides, sulfonamides, fluoroquinolones, and lincosamides. The matrix-matched calibration technique with internal standard addition for each antibiotic group was employed for quantification. Two extractions employing an Oasis HLB cartridge and two injections were needed for each sample due to the different physico-chemical properties of the tested antibiotics. Prior to the extraction on the Oasis HLB SPE cartridge, the samples were adjusted to pH 4.5 and 7.5. The usefulness and versatility of the method was documented by achieving method limits of quantification up to 10 ng L-1 and recoveries >80% for most analytes Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2014
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