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Aster Yellows Subgroup 16SrI-C Phytoplasma in Rhododendron hybridum

SYSNO ASEP	0395153
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Aster Yellows Subgroup 16SrI-C Phytoplasma in Rhododendron hybridum
Author(s)	Přibylová, Jaroslava (BC-A)_{RID, ORCID} Špak, Josef (BC-A)_{RID, ORCID} Fránová, Jana (BC-A)_{RID, ORCID}
Source Title	Journal of Phytopathology - Phytopathologische Zeitschrift. - : Wiley - ISSN 0931-1785 Roč. 161, 7-8 (2013), s. 590-593
Number of pages	4 s.
Publication form	Print - P
Language	eng - English
Country	DE - Germany
Keywords	electron microscopy ; PCR ; phytoplasma
Subject RIV	EE - Microbiology, Virology
R&D Projects	LD12074 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	BC-A - RVO:60077344
UT WOS	000321507600019
DOI	10.1111/jph.12099
Annotation	Symptoms of unknown aetiology on Rhododendron hybridum were observed in the Czech Republic in 2010. The infected plant had malformed leaves, with irregular shaped edges, mosaic, leaf tip necrosis and multiple axillary shoots with smaller leaves. Transmission electron microscopy showed phytoplasma-like bodies in phloem cells of the symptomatic plant. Phytoplasma presence was confirmed by PCR using phytoplasma-specific, universal and group-specific primer pairs. RFLP analysis of 16S rDNA enabled classification of the detected phytoplasma into the aster yellows subgroup I-C. Sequence analysis of the 16S-23S ribosomal operon of the amplified phytoplasma genome from the infected rhododendron plant (1724bp) confirmed the closest relationship with the Czech Echinacea purpurea phyllody phytoplasma. These data suggest Rhododendron hybridum is a new host for the aster yellows phytoplasma subgroup 16SrI-C in the Czech Republic and worldwide.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2014

Number of the records: 1