Number of the records: 1
Differential freshwater flagellate community response to bacterial food quality with a focus on Limnohabitans bacteria
- 1.
SYSNO ASEP 0394406 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Differential freshwater flagellate community response to bacterial food quality with a focus on Limnohabitans bacteria Author(s) Šimek, Karel (BC-A) RID, ORCID
Kasalický, Vojtěch (BC-A) RID, ORCID
Jezbera, Jan (BC-A) RID
Horňák, Karel (BC-A) RID
Nedoma, Jiří (BC-A) RID, ORCID
Hahn, M.W. (AT)
Bass, D. (GB)
Jost, S. (DE)
Boenigk, J. (DE)Source Title The ISME Journal. - : Nature Publishing Group - ISSN 1751-7362
Roč. 7, č. 8 (2013), s. 1519-1530Number of pages 12 s. Language eng - English Country GB - United Kingdom Keywords flagellate community composition ; food quality of bacteria ; Limnohabitans ; 454 pyrosequencing ; freshwater ; flagellate growth Subject RIV DA - Hydrology ; Limnology R&D Projects GA13-00243S GA ČR - Czech Science Foundation (CSF) EE2.3.30.0032 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BC-A - RVO:60077344 UT WOS 000322119600006 EID SCOPUS 84880916344 DOI 10.1038/ismej.2013.57 Annotation Different bacterial strains can have different value as food for heterotrophic nanoflagellates (HNF), thus modulating HNF growth and community composition. We examined the influence of prey food quality using four Limnohabitans strains, one Polynucleobacter strain and one freshwater actinobacterial strain on growth (growth rate, length of lag phase and growth efficiency) and community composition of a natural HNF community from a freshwater reservoir. Pyrosequencing of eukaryotic small subunit rRNA amplicons was used to assess time-course changes in HNF community composition. All four Limnohabitans strains and the Polynucleobacter strain yielded significant HNF community growth while the actinobacterial strain did not although it was detected in HNF food vacuoles. Notably, even within the Limnohabitans strains we found significant prey-related differences in HNF growth parameters, which could not be related only to size of the bacterial prey. Sequence data characterizing the HNF communities showed also that different bacterial prey items induced highly significant differences in community composition of flagellates. Generally, Stramenopiles dominated the communities and phylotypes closely related to Pedospumella (Chrysophyceae) were most abundant bacterivorous flagellates rapidly reacting to addition of the bacterial prey of high food quality. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2014
Number of the records: 1