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Optimized method for identification of the proteomes secreted by cardiac cells
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SYSNO ASEP 0392231 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Optimized method for identification of the proteomes secreted by cardiac cells Author(s) Šťastná, Miroslava (UIACH-O) RID, ORCID
Van Eyk, J.E. (US)Number of authors 2 Source Title Methods in molecular biology - ISSN 1940-6029
Roč. 1005, č. 1005 (2013), s. 225-235Number of pages 11 s. Publication form Print - P Language eng - English Country US - United States Keywords cardiac cells ; secreted proteins ; proteomic technology Subject RIV CB - Analytical Chemistry, Separation Institutional support UIACH-O - RVO:68081715 DOI 10.1007/978-1-62703-386-2_18 Annotation There are several crucial steps that have to be followed to ensure successful secreted proteome detection and identification. Simultaneously with the optimization of the experimental conditions for various cell type culturing and subsequent cell conditioning to obtain conditioned medium with secreted proteins in vitro, the analytical separation methods for fractionation of complex protein mixture and mass spectrometry for protein identification are of high importance. Although optimized for neonatal cardiac myocytes, the general steps described in the following chapter can be adapted to other cell types as well. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2014
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