Number of the records: 1

Glycoproteins and protein glycations identified in barley grain and malt by 2D-HPLC

SYSNO ASEP	0391056
Document Type	A - Abstract
R&D Document Type	The record was not marked in the RIV
R&D Document Type	Není vybrán druh dokumentu
Title	Glycoproteins and protein glycations identified in barley grain and malt by 2D-HPLC
Author(s)	Žídková, Jitka (UIACH-O) Petry-Podgórska, Inga (UIACH-O) Laštovičková, Markéta (UIACH-O)_{RID, ORCID} Bobálová, Janette (UIACH-O)_{RID, ORCID}
Number of authors	4
Source Title	Materials Structure in Chemistry, Biology, Physics and Technology. - : Czech and Slovak Crystallographic Association - ISSN 1211-5894 Roč. 20, č. 1 (2013), s. 43
Number of pages	1 s.
Publication form	Print - P
Action	Discussion in Structural Molecular Biology /11./
Event date	14.03.2013-16.03.2013
VEvent location	Nové Hrady
Country	CZ - Czech Republic
Event type	EUR
Language	eng - English
Country	CZ - Czech Republic
Keywords	barley grain ; glycoproteins ; 2D-HPLC ; MS/MS
Subject RIV	CB - Analytical Chemistry, Separation
R&D Projects	GPP503/12/P395 GA ČR - Czech Science Foundation (CSF)
Institutional support	UIACH-O - RVO:68081715
Annotation	Two-dimensional liquid chromatography (ion-exchange and reversed-phase) with off-line mass-spectrometric detection (MALDI-TOF-MS/MS) has been used to analyze glycoproteins and glycated proteins in barley grain and malt. Seven glycoproteins were identified by partial sequencing based on fragmentation MS/MS spectra. Glycations were observed for five out of 16 unambiguously assigned proteins in malt extract, while no glycation was detected in barley grains. The results document that the proposed analytical procedure is well suited for monitoring carbohydrate-modified proteins in cereal products and their end foodstuff products.
Workplace	Institute of Analytical Chemistry
Contact	Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234
Year of Publishing	2013

Number of the records: 1