Efficient disruption of endogenous Bombyx gene by TAL effector nucleases

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SYSNO ASEP	0390514
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Efficient disruption of endogenous Bombyx gene by TAL effector nucleases
Author(s)	Sajwan, Suresh (BC-A) Takasu, Y. (JP) Tamura, T. (JP) Uchino, K. (JP) Sezutsu, H. (JP) Žurovec, Michal (BC-A)_{RID, ORCID}
Number of authors	6
Source Title	Insect Biochemistry and Molecular Biology. - : Elsevier - ISSN 0965-1748 Roč. 43, č. 1 (2013), s. 17-23
Number of pages	7 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	gene targeting ; ZFN ; nonhomologous end joining
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GAP305/10/2406 GA ČR - Czech Science Foundation (CSF)
Institutional support	BC-A - RVO:60077344
UT WOS	000313934800003
DOI	10.1016/j.ibmb.2012.10.011
Annotation	TALENS are engineered nucleases used for targeted gene disruptions in various organisms. We examined the potential of using full length TALENs for site-specific mutagenesis in silkworm. Three full-length TALEN pairs were synthesized by Golden gate assembly, used for activity examination in heterologous yeast system and finally for mutagenesis in Bombyx mori germline. All TALENs were able to generate somatic and germline mutations in silkworms albeit at low frequency.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2013
Electronic address	http://www.sciencedirect.com/science/article/pii/S0965174812001622#

Number of the records: 1