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Atomic Force Microscopy Investigations into Biology - From Cell to Protein
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SYSNO ASEP 0390150 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Artifacts in atomic force microscopy of biological samples Author(s) Ukraintsev, Egor (FZU-D) RID, ORCID
Kromka, Alexander (FZU-D) RID, ORCID, SAI
Kozak, Halyna (FZU-D) RID, ORCID
Remeš, Zdeněk (FZU-D) RID, ORCID
Rezek, Bohuslav (FZU-D) RID, ORCIDSource Title Atomic Force Microscopy Investigations into Biology - From Cell to Protein. - Rijeka : InTech, 2012 / Frewin C.L. - ISBN 978-953-51-0114-7 Pages s. 29-54 Number of pages 26 s. Number of pages 354 Publication form Print - P Language eng - English Country HR - Croatia Keywords atomic force microscopy (AFM) ; validity of measurement ; accuracy of measurement Subject RIV BM - Solid Matter Physics ; Magnetism R&D Projects LC06040 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) KAN400100701 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) LC510 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z10100521 - FZU-D (2005-2011) DOI 10.5772/2092 Annotation Atomic force microscopy (AFM) is a frequently used method applicable also to biological studies due to its capability of measuring in native environment. However, AFM images often contain features which are not present on the sample in reality, but are a direct result of the measurements itself. Such structures or features are denoted as artifacts. The artifacts arise from various reasons. Some of them can be avoided during measurements, other ones are inherent. The artifacts shown in this chapter are divided into several categories based on the part of AFM which is responsible for them. Examples of many artifacts are presented and their reasons are explained. This chapter will be helpful for AFM users who observe strange effects, presumably related to the AFM technique, but do not know their reason. This chapter can also answer questions about the validity of imaged features as well as their accuracy. Workplace Institute of Physics Contact Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579 Year of Publishing 2013
Number of the records: 1