Number of the records: 1
Lipid alterations in human colon epithelial cells induced to differentiation and/or apoptosis by butyrate and polyunsaturated fatty acids
- 1.
SYSNO ASEP 0389673 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Lipid alterations in human colon epithelial cells induced to differentiation and/or apoptosis by butyrate and polyunsaturated fatty acids Author(s) Hofmanová, Jiřina (BFU-R) RID, ORCID
Ciganek, M. (CZ)
Slavík, J. (CZ)
Kozubík, Alois (BFU-R) RID, ORCID
Stixová, Lenka (BFU-R) RID, ORCID
Vaculová, Alena (BFU-R) RID, ORCID
Dušek, L. (CZ)
Machala, M. (CZ)Number of authors 8 Source Title Journal of Nutritional Biochemistry. - : Elsevier - ISSN 0955-2863
Roč. 23, č. 6 (2012), s. 539-548Number of pages 10 s. Publication form Print - P Language eng - English Country US - United States Keywords Colon cancer ; Polyunsaturated fatty acids ; Butyrate Subject RIV BO - Biophysics R&D Projects GA524/07/1178 GA ČR - Czech Science Foundation (CSF) GAP301/11/1730 GA ČR - Czech Science Foundation (CSF) Institutional support BFU-R - RVO:68081707 CEZ AV0Z50040507 - BFU-R (2005-2011) UT WOS 000304786000004 DOI 10.1016/j.jnutbio.2011.02.010 Annotation The present study highlights the important association between lipid alterations and differentiation/apoptotic responses in human colon differentiating (FHC) and nondifferentiating (HCT-116) cell lines after their treatment with short-chain fatty acid sodium butyrate (NaBt), polyunsaturated fatty acids (PUFAs), and/or their combination. Our data from GC/MS and LC/MS/MS showed an effective incorporation and metabolization of the supplemented arachidonic acid (AA) or docosahexaenoic acid (DNA), resulting in an enhanced content of the respective PUFA in individual phospholipid (PL) classes and an altered composition of the whole cellular fatty acid spectrum in both FHC and HCT-116 cells. We provide novel evidence that NaBt combined with PUFAs additionally modulated AA and DHA cellular levels and caused their shift from triacylglycerol to PL fractions. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2013
Number of the records: 1