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Synthetic Peptides Analogue to Enamel Proteins Promote Osteogenic Differentiation of MC3T3-E1 and Mesenchymal Stem Cells
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SYSNO ASEP 0389610 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Synthetic Peptides Analogue to Enamel Proteins Promote Osteogenic Differentiation of MC3T3-E1 and Mesenchymal Stem Cells Author(s) Rubert, M. (ES)
Ramis, J. M. (ES)
Vondrášek, Jiří (UOCHB-X) RID, ORCID
Gaya, A. (ES)
Lyngstadaas, S. P. (NO)
Monjo, M. (ES)Number of authors 6 Source Title Journal of Biomaterials and Tissue Engineering - ISSN 2157-9083
Roč. 1, č. 2 (2011), s. 198-209Number of pages 12 s. Language eng - English Country US - United States Keywords proline-rich regions ; synthetic peptides ; bone formation ; mineralization ; In Vitro Subject RIV EI - Biotechnology ; Bionics CEZ AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000312570600009 DOI 10.1166/jbt.2011.1018 Annotation Regulation of biomineralization processes are mediated by extracellular matrix proteins that often exhibit proline-rich regions. Advanced bioinformatic methods were used to design the structure of artificial peptides (P1, P2, P3) based on proline-rich domains of extracellular proteins. Their effect on osteoblast differentiation and in vitro biomineralization was tested on MC3T3-E1 and human umbilical cord mesenchymal stem cells (hUCMSCs) and compared to the commercially available enamel matrix derivative (EMD). MC3T3-E1 and hUCMSCs treated with the synthetic peptides showed a decreased cytotoxicity after 24-48 h of treatment compared to control. MC373-E1 cells treated with EMD showed lower expression of osteoblast markers genes than cells treated with P2, except for collagen type I. In hUCMSCs, OC gene expression was higher in P2-treated cells compared to those treated with EMD or control. ALP activity was markedly increased in MC3T3-E1 cells incubated with P2 compared to other treatments. Similar results were observed in hUCMSCs. Further, P2 increased calcium deposition rate compared to EMD or control either in MC3T3-E1 or hUCMSCs. The observed effects of proline-rich peptides hold potential for both clinical applications and as a research tool in further investigations of the molecular basis of induced osteogenic cell differentiation. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Year of Publishing 2013
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