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Enzyme Hydrolysis of Soybean Oil in a Slug Flow Microsystem
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SYSNO ASEP 0388303 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Enzyme Hydrolysis of Soybean Oil in a Slug Flow Microsystem Author(s) Čech, J. (CZ)
Schrott, W. (CZ)
Slouka, Z. (CZ)
Přibyl, M. (CZ)
Broz, M. (CZ)
Kuncová, Gabriela (UCHP-M) RID, SAI
Šnita, D. (CZ)Source Title Biochemical Engineering Journal. - : Elsevier - ISSN 1369-703X
Roč. 67, AUG 15 (2012), s. 194-202Number of pages 9 s. Language eng - English Country CH - Switzerland Keywords lipase ; microemulsion ; micromixing Subject RIV EI - Biotechnology ; Bionics R&D Projects ME 892 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UCHP-M - RVO:67985858 UT WOS 000310945100025 DOI 10.1016/j.bej.2012.06.015 Annotation In this paper, we report on the development of a continuous microfluidic reaction system for hydrolysis of soybean oil catalyzed with Thermomyces lanuginosus lipase (Lipolase 100 L). The microfluidic reaction system generates water-oil emulsions in the form of a hydrodynamically well controlled slug flow and automatically separates the oil phase after the hydrolysis by employing two microfluidic separators. All elements of this reaction system were tested at different hydrodynamic conditions and showed the ability to operate in a wide range of reactant flow rates. 25-30% conversion of triglyceride was reached in setting the residence time of the emulsion mixture to 10 min. This conversion increased to almost 50% for the residence time of 1 h. These results are comparable with those published for the same enzyme reaction system. This feature along with the benefits stemming from the use of microfluidics make our developed system a useful, easy to control and easy to scale-up technology for fast production of fine chemicals. Moreover, our calculations indicate that our slug flow system allows for significant savings of the mechanical energy. Workplace Institute of Chemical Process Fundamentals Contact Eva Jirsová, jirsova@icpf.cas.cz, Tel.: 220 390 227 Year of Publishing 2013
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