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The actin family member Arp6 and the histone variant H2A.Z are required for spatial positioning of chromatin in chicken cell nuclei
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SYSNO ASEP 0387825 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The actin family member Arp6 and the histone variant H2A.Z are required for spatial positioning of chromatin in chicken cell nuclei Author(s) Maruyama, E.O. (JP)
Hori, T. (JP)
Tanabe, H. (JP)
Kitamura, H. (JP)
Matsuda, R. (JP)
Tone, S. (JP)
Hozák, Pavel (UMG-J) RID, ORCID
Habermann, F.A. (DE)
von Hase, J. (DE)
Cremer, C. (DE)
Fukagawa, T. (JP)
Harata, M. (JP)Source Title Journal of Cell Science. - : Company of Biologists - ISSN 0021-9533
Roč. 125, č. 16 (2012), s. 3739-3744Number of pages 6 s. Language eng - English Country GB - United Kingdom Keywords actin-related protein ; histone variant ; nuclear organization Subject RIV EB - Genetics ; Molecular Biology R&D Projects LC545 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LH12143 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UMG-J - RVO:68378050 UT WOS 000309525300006 DOI 10.1242/jcs.103903 Annotation The spatial organization of chromatin in the nucleus contributes to genome function and is altered during the differentiation of normal and tumorigenic cells. Although nuclear actin-related proteins (Arps) have roles in the local alteration of chromatin structure, it is unclear whether they are involved in the spatial positioning of chromatin. In the interphase nucleus of vertebrate cells, gene-dense and gene-poor chromosome territories (CTs) are located in the center and periphery, respectively. We analyzed chicken DT40 cells in which Arp6 had been knocked out conditionally, and showed that the radial distribution of CTs was impaired in these knockout cells. Arp6 is an essential component of the SRCAP chromatin remodeling complex, which deposits the histone variant H2A.Z into chromatin. The redistribution of CTs was also observed in H2A. Z-deficient cells for gene-rich microchromosomes, but to lesser extent for gene-poor macrochromosomes. These results indicate that Arp6 and H2A.Z contribute to the radial distribution of CTs through different mechanisms. Microarray analysis suggested that the localization of chromatin to the nuclear periphery per se is insufficient for the repression of most genes. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2013
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