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Bioluminescence determination of active caspase-3 in single apoptotic cells

SYSNO ASEP	0387334
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Bioluminescence determination of active caspase-3 in single apoptotic cells
Author(s)	Lišková, Marcela (UIACH-O) Klepárník, Karel (UIACH-O)_{RID, ORCID} Matalová, Eva (UZFG-Y)_RID Hegrová, Jitka (UIACH-O) Přikryl, Jan (UIACH-O)_{RID, ORCID} Švandová, Eva (UZFG-Y)_{RID, ORCID} Foret, František (UIACH-O)_{RID, ORCID}
Number of authors	7
Source Title	Electrophoresis. - : Wiley - ISSN 0173-0835 Roč. 34, č. 12 (2013), s. 1772-1777
Number of pages	6 s.
Publication form	Print - P
Language	eng - English
Country	DE - Germany
Keywords	apoptosis ; bioluminescence ; caspase-3
Subject RIV	CB - Analytical Chemistry, Separation
R&D Projects	GAP206/11/2377 GA ČR - Czech Science Foundation (CSF)
Institutional support	UIACH-O - RVO:68081715 ; UZFG-Y - RVO:67985904
UT WOS	000320385400009
EID SCOPUS	84879141364
DOI	10.1002/elps.201200675
Annotation	In this work we report on the development of a high-sensitivity instrumentation and protocol for detection of active caspase-3 in individual mammalian apoptotic cells. The technology is based on the specific cleavage of modified luciferin by caspase-3, an immediate bioluminescence reaction of free luciferin with luciferase followed by emissions of photons and their detection by photomultiplier tube working in the photon counting regime.
Workplace	Institute of Analytical Chemistry
Contact	Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234
Year of Publishing	2014

Number of the records: 1