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Open-tubular capillary electrochromatography with bare gold nanoparticles-based stationary phase applied to separation of trypsin digested proteins

  1. 1.
    SYSNO ASEP0387211
    Document TypeC - Proceedings Paper (int. conf.)
    R&D Document TypeThe record was not marked in the RIV
    TitleOpen-tubular capillary electrochromatography with bare gold nanoparticles-based stationary phase applied to separation of trypsin digested proteins
    Author(s) Mikšík, Ivan (FGU-C) RID, ORCID, SAI
    Lacinová, Kateřina (FGU-C) RID
    Zmatlíková, Zdeňka (FGU-C)
    Sedláková, Pavla (FGU-C) RID
    Král, V. (CZ)
    Sýkora, D. (CZ)
    Řezanka, P. (CZ)
    Kašička, Václav (UOCHB-X) RID, ORCID
    Number of authors8
    Source TitleActa Universitatis Palackianae Olomucensis. Facultas Rerum Naturalium. Chemica 50S. - Olomouc : Faculty of Science, Palacký University Olomouc, 2012 / Maier V. ; Ševčík J. - ISSN 0232-0061 - ISBN 978-80-244-3115-4
    Pagess. 16-17
    Number of pages2 s.
    ActionInternational Symposium Advances in Chromatography and Electrophoresis and Chiranal
    Event date11.06.2012-14.06.2012
    VEvent locationOlomouc
    CountryCZ - Czech Republic
    Event typeEUR
    Languageeng - English
    CountryCZ - Czech Republic
    Keywordsopen-tubular capillary electrochromatography ; gold nanoparticles ; glycation of proteins
    Subject RIVCB - Analytical Chemistry, Separation
    R&D ProjectsGA203/09/0675 GA ČR - Czech Science Foundation (CSF)
    GAP206/12/0453 GA ČR - Czech Science Foundation (CSF)
    CEZAV0Z50110509 - FGU-C (2005-2011)
    AV0Z40550506 - UOCHB-X (2005-2011)
    AnnotationOpen-tubular capillary electrochromatography (OT-CEC) using bare gold nanoparticles-based stationary phase has been applied to separation of tryptic peptide fragments of native and glycated proteins, bovine serum albumin and human transferrin. OT-CEC with bare gold nanoparticles stationary phase was found to be a suitable technique for separation of complex peptide mixtures originating from enzymatic (tryptic) digestion of native and glycated bovine serum albumin and human transferrin, and for glycation studies of these proteins.
    WorkplaceInstitute of Organic Chemistry and Biochemistry
    Contactasep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418
    Year of Publishing2013
Number of the records: 1  

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